白藜芦醇对阿司咪唑诱导斑马鱼心脏损伤的保护作用  

Protection effect of resveratrol to astemizole-induced cardiac injury of zebrafish embryos

作　　者：薛迪[1,2] 韩利文[2] 杨继芳 何秋霞[2] 王雪[2] 侯海荣[2] 张爱平[1] 刘可春[2] 

机构地区：[1]山西医科大学药学院,山西太原030001 [2]山东省科学院生物研究所山东省生物传感器重点实验室,山东济南250014 [3]青岛开发区第一人民医院护理部,山东青岛266555

出　　处：《山东科学》2015年第3期29-33,共5页Shandong Science

基　　金：国家自然科学基金青年基金(81202584;31400979);山东省自主创新成果转化重大专项(2014ZZCX0215);山东省中医药科学技术研究项目(2013-173)

摘　　要：探讨不同浓度的白藜芦醇对阿司咪唑诱导斑马鱼胚胎心脏损伤的保护作用。采用发育正常的48 hpf斑马鱼胚胎作为实验模型,加入阿司咪唑(4μmol/L)后再加入不同浓度的白藜芦醇处理上述胚胎,于72 hpf观察胚胎心脏的形态、心率及静脉窦-动脉球(SV-BA)的变化。结果发现阿司咪唑模型对照组在给药后出现心脏损伤现象,包括心包水肿、心脏周围血细胞堆积、心率减慢和SV-BA间距的缩短,白藜芦醇给药组对上述损伤情况改善明显,并且随着给药浓度的增加,阿司咪唑诱导的心脏毒性作用明显减小。研究认为白藜芦醇对阿司咪唑诱导的斑马鱼心脏毒性有一定的保护作用。We explore the protection effect of different concentrations resveratrol to astemizole-induced cardiac injury of zebrafish embryos. We treat zebrafish embryos with 4 μmol/L astemizole and different concentrations resveratrol with normal growth 48 hpf zebrafish embryo as an experimental model. We observe cardiac phenotypes, heart rates and the variation of SV-BA at 72 hpf. Astemizole group shows heart injury such as pericardium edema, blood cell accumulation around the heart, heart rate reduction and SV-BA interval shortening after drug feeding. However, astemizole-induced heart toxicity significantly decreases with the increase of resveratrol concentration for resveratrol group. We therefore think resveratrol can protect astemizole-induced cardiac injury of zebrafish embryos.

关 键 词：斑马鱼 心脏损伤 心率 阿司咪唑 白藜芦醇 保护作用 

分 类 号：R965.1[医药卫生—药理学]