膈下逐瘀汤加减方作用人肝癌细胞SMMC-7721蛋白质组学研究  

The proteomics research of human hepatic carcinoma cell line SMMC-7721 on the effects of Gexia Zhuyu decoction pharmacological serum

作　　者：李华成 王建刚[2] 费新应 汪建平[3] 

机构地区：[1]黄石市中医医院(传染病医院)肝病科,湖北黄石435100 [2]湖北中医药大学,湖北武汉430065 [3]华中科技大学同济医学院药学院,湖北武汉430030

出　　处：《中西医结合肝病杂志》2015年第3期156-158,172,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然科学基金项目(No.31270395);湖北省自然科学基金项目(No.2011CDB170)

摘　　要：目的:运用蛋白质组学的研究方法,分析膈下逐瘀汤加减方(GXZY)含药血清作用人肝癌细胞SMMC-7721的差异蛋白质表达情况,以期寻找GXZY抗肿瘤作用靶点。方法:将人肝癌细胞SMMC-7721传代培养后,随机分为GXZY血清组以及空白对照组,应用双向凝胶电泳、MALDI-TOF-MS鉴定分析差异蛋白质表达情况。结果:GXZY血清组和空白对照组的蛋白电泳图谱上各有33个蛋白质点高表达,经质谱分析鉴定出6个蛋白质点,GXZY血清组有5个蛋白质点AHCY、PGAM1、STMN1、HSP90AB1、VAT-1表达下调,1个蛋白质点TPM4表达上调。结论:GXZY含药血清处理的人肝癌细胞SMMC-7721蛋白质质谱表达有差异,这些差异蛋白质可能是GXZY治疗的作用靶点。Objective： To analyze the differential expression protein of human hepatic carcinoma cell line SMMC-7721 under the Gexia Zhuyu decoction（ GXZY） pharmacological serum,in order to find the anti-tumor target of GXZY. Methods： Subcultured human hepatic carcinoma cell line SMMC-7721 were randomly divided into GXZY pharmacological serum groups and blank drug serum group,using two-dimensional gel electrophoresis,MALDI-TOF-MS to analyze the differential expression protein. Results： High expression of 33 protein spots was found in each of the groups,6 proteins were identified by mass spectrometry analysis. Expression of 5 protein spots in GXZY pharmacological serum group decreased（ AHCY,PGAM1,STMN1,HSP90AB1,VAT1） and 1 increased（ TPM4）. Conclusion： There are differential expression proteins of human hepatic carcinoma cell line SMMC-7721 in GXZY pharmacological serum,these differential expression proteins may be the therapeutic targets of GXZY.

关 键 词：膈下逐瘀汤/药理学 蛋白质组学 人肝癌细胞SMMC-7721 

分 类 号：R285[医药卫生—中药学]