干酪乳杆菌Lactobacillus casei 393 upp基因突变株的构建  

Construction of an Lactobacillus Casei 393 upp mutant

作　　者：郭建军[1] 袁林[1] 曾静[1] 付锦楠[1] 龚小华[1] 何顺华[1] 

机构地区：[1]江西省科学院微生物研究所,南昌330096

出　　处：《江西科学》2015年第3期308-311,共4页Jiangxi Science

基　　金：江西省科技支撑计划项目(20142BBF60036);江西省自然(青年)科学基金项目(2013BAB214014)

摘　　要：upp基因可用来作为反向筛选标记基因。利用p ORI温度敏感型双质粒系统敲除干酪乳杆菌Lactobacillus casei 393的upp基因,构建得到重组菌Lactobacillus casei 393-△upp。重组菌可作为反向筛选标记基因upp使用的宿主菌。Upp gene exists widely in prokaryotes and some primary eukaryotes cells. UPRT （ Uracil Phosphoribosyl transferase ）, eneoded by upp gene, can convert 5-fluorouracil to 5-fluoro-dUMP, which inhibits thymidylate synthase, causing cell death. So the upp gene can be used as a eounterse- lectable marker. In this study, we construst Lactobacillus casei 393-△upp mutant by pORI tempera- ture-sensitive dual plasmid system and the upp counterselectable marker can be used in this upp de- leted strain.

关 键 词：干酪乳杆菌 upp基因 突变株 

分 类 号：Q936[生物学—微生物学]