Theoretical Basis of Gene Splitting Technique and Its Application in the Control of Transgene Flow  

作　　者：Yufeng DONG Xujing WANG Qiaoling TANG Zhixing WANG 

机构地区：[1]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences

出　　处：《Agricultural Biotechnology》2015年第3期1-4,共4页农业生物技术（英文版）

基　　金：Supported by Major Project of China on New Varieties of GMO Cultivation(2013zx08010-003);National Natural Science Foundation of China(31100408)

摘　　要：Transgenic safety issues cause more and more controversies with the planting area of transgenie crops increased year by year. Gene flow from transgenie crops to wild relatives through pollen dispersal is one of the focus problems. Gene splitting technique provides a new strategy for the control of transgene flow by bio-logical containment. The construction of gene splitting technique is based on protein trans-splicing mediated by intein. Currently, it has been proved in Arabidopsis, tabaoco, wheat, etc. that active and functional proteins can be reassembled by intein mediated protein trans-splicing after gene splitting, which provides theoretical basis and experimental supporting for the limit of transgene flow by gene splitting technique. The theoretical basis of gene splitting technique and research progresses of its application on the control of transgene flow were reviewed in this paper.Transgenic safety issues cause more and more controversies with the planting area of transgenie crops increased year by year. Gene flow from transgenie crops to wild relatives through pollen dispersal is one of the focus problems. Gene splitting technique provides a new strategy for the control of transgene flow by bio-logical containment. The construction of gene splitting technique is based on protein trans-splicing mediated by intein. Currently, it has been proved in Arabidopsis, tabaoco, wheat, etc. that active and functional proteins can be reassembled by intein mediated protein trans-splicing after gene splitting, which provides theoretical basis and experimental supporting for the limit of transgene flow by gene splitting technique. The theoretical basis of gene splitting technique and research progresses of its application on the control of transgene flow were reviewed in this paper.

关 键 词：Gene splitting INTEIN Gene flow 

分 类 号：Q943.2[生物学—植物学]