磷酸肌酸对大鼠子宫缺血再灌注损伤的影响  

Effect of Phosphocreatine on Uterine Ischemia-reperfusion Injury in Rats

作　　者：吕丽贤[1] 张陈彦[1] 安雪丽 张铭娜[1] 张立新[1] 

机构地区：[1]石家庄市第一医院产科,石家庄050011

出　　处：《解放军医药杂志》2015年第5期26-28,共3页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：石家庄市科技指导计划(141462453)

摘　　要：目的探讨外源性磷酸肌酸预处理对大鼠子宫缺血再灌注损伤的保护作用及其机制。方法选择SD雌性大鼠45只,随机分为假手术组、缺血再灌注组和干预组,每组15只。干预组于缺血前30 min经腹腔注射磷酸肌酸注射液10 mg/kg,测定子宫组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平,并观察子宫病理组织学改变。结果与假手术组比较,缺血再灌注组和干预组大鼠子宫组织SOD活性明显降低,MDA含量明显增加(P<0.05);与缺血再灌注组比较,干预组SOD活性升高,MDA含量降低(P<0.05)。干预组病理组织学观察炎性反应较缺血再灌注组明显减轻。结论磷酸肌酸对大鼠子宫缺血再灌注损伤有保护作用,其机制可能与其增强SOD活性有关。Objective To study the protective effect and its mechanisms of exogenous creatine phosphate（PCr）preconditioning on uterine ischemia-reperfusion injury in rats. Methods A total of 45 SD female rats were randomly divided into sham operation group（ n = 15）,ischemia-reperfusion group（ n = 15） and intervention group（ n = 15）. The intervention group was given 10 mg / kg PCr injection via intraperitoneal injection 30 min before ischemia. The activity of superoxide dismutase（ SOD） and malondialdehyde（ MDA） level in uterine tissues were detected,and the pathological changes of uterus were also observed. Results Compared with those in the sham operation group,the SOD activities were significantly reduced,while MDA levels were significantly increased in ischemia-reperfusion and intervention groups（P〈0. 05）; compared with those in ischemia-reperfusion group,the SOD activity was significantly increased,and MDA level was significantly reduced in the intervention group（ P〈0. 05）. Histopathological observation showed that inflammatory reaction in the intervention group was significantly alleviated compared with that in the ischemia-reperfusion group.Conclusion PCr may protect uterine ischemia-reperfusion injury to a certain degree in rats,and the mechanisms may be related with its activation to SOD activity.

关 键 词：磷酸肌酸 再灌注损伤 子宫 大鼠 Sprague-Dawley 

分 类 号：R965[医药卫生—药理学] R-332[医药卫生—药学]