电针“百会”、“涌泉”两穴对APP/PS1双转基因小鼠学习记忆行为与海马超微结构的影响  

The effect of electro-acupuncture at Baihui and Yongquan on the hippocampus ultrastructure in APP/PS1 double transgenic mice

作　　者：加吾拉.阿不力孜 王鑫[1] 李芙[1] 白杨[1] 姚海江[1] 莫雨平[1] 周源[1] 许红[2] 毛颖秋[3] 高誉珊[2] 张伟[3] 张忠[2] 李志刚[1] 薛卫国[1] 

机构地区：[1]北京中医药大学针灸推拿学院,博士研究生100029 [2]北京中医药大学基础医学院,100029 [3]北京中医药大学科研实验中心,100029

出　　处：《环球中医药》2015年第4期389-394,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81273826)

摘　　要：目的观察电针对淀粉样前蛋白/早老蛋白1(amyloid precursor protein/presenilin-1,APP/PS1)双转基因鼠空间学习记忆行为及海马超微结构的影响。方法将4月龄的APP/PS1双转基因小鼠按随机分配法分为模型组、电针组和药物组,以相同月龄同窝阴性小鼠作为对照组,每组12只。电针组取"百会"、"涌泉"两穴,每次留针时间为15分钟,隔日1次,针刺5周;药物组予乙酰胆碱酶抑制剂0.92 m L/g灌胃。Morris水迷宫检测小鼠学习记忆能力及空间探索能力,取脑做海马CA1区透射电镜片并观察。结果 Morris水迷宫检测四天小鼠上台潜伏期结果显示:模型组与对照组比较有统计学差异(P<0.05);空间探索实验:模型组在原有平台象限(第三象限)区域活动时间明显低于对照组(P<0.05);透射电镜结果显示:在微血管、神经元突触及神经元超微结构上对照组优于模型组。结论电针治疗可改善APP/PS1双转基因小鼠学习记忆能力,并对海马超微结构有良性调节作用,这些表现可能是电针改善AD小鼠学习记忆行为学的机制之一。Objective To observe the effect of electro-acupuncture（ EA） on the abilities of spatial learning andmemory and the hippocampus ultrastructure in APP / PS1 double transgenic mice. Methods APP / PS1 double transgenic mice（ 4-month-old） are divided into model group,EA group and drug（ ACh E）group according to the random assignment method,age-matched one brood mice with negative transgenes were used as control group,with 12 mice in each group. For the EA group,EA was applied to Baihui（ GV20） and Yongquan（ KI 1） for 15 min,once every other day for 5 weeks. For the drug group,0. 92 ml / g of acetylcholine enzyme was given by intragastricadministration. The learning-memory ability and space exploration ability of the mice were tested by using Morris water maze. Remove the brains to make and observe the hippocampal CA 1 area transmission electric lens. Results The escape latency periods of the mice were detected by Morris water maze for four days,the result showed that there was a statistical difference between the model group and the control group（ P〈0. 05）; space exploration experiment revealed that the activity time of the model group in the original platform quadrant（ the third quadrant） is significantly lower than that of the control group（ P〈0. 05）; transmission electron microscopy results showed that the control group is superior to the model group in micro-vessels,neuron synapses and ultrastructure of neurons. Conclusion EA therapy can improve the learning-memory ability of the APP / PS1 double transgenic mice and positively regulate the hippocampus ultrastructure,which might be one of the mechanisms for EA to improve the learning-memory behavior of AD mice.

关 键 词：阿尔茨海默症 淀粉样前蛋白/早老蛋白1 海马 超微结构 电针 

分 类 号：R245.3[医药卫生—针灸推拿学]