众生丸对H1N1流感小鼠的保护及免疫调节作用研究  

Protection and immunoregulation of Zhongsheng Pill on mice infected with H1N1 virus

作　　者：戴卫平[1] 李耿[1] 申小花[1] 吴依娜[1] 张奉学[2] 赖小平[3] 

机构地区：[1]广州中医药大学中药学院,硕士研究生510006 [2]广州中医药大学热带医学研究所 [3]东莞广州中医药大学中医药数理工程研究院

出　　处：《环球中医药》2015年第4期395-399,共5页Global Traditional Chinese Medicine

基　　金：广东省自然科学基金(S2012030006598);广东省科技计划(2010A032100006);东莞市科技局项目(2011108102048)

摘　　要：目的研究众生丸对H1N1流感病毒感染小鼠的保护及免疫调节作用。方法以奥司他韦为阳性药,H1N1流感病毒滴鼻感染小鼠复制小鼠病毒性肺炎模型。比较小鼠病毒性肺炎模型的肺指数、肺组织形态学、死亡保护率、小鼠T淋巴细胞亚群和细胞因子干扰素-γ(Interferon-γ,IFN-γ)、白细胞介素-10(interleukin-10,IL-10)的数量变化。结果与病毒对照组比较,众生丸可显著降低H1N1感染小鼠的肺指数,提高存活率,调节T细胞亚群含量,促进血清中IFN-γ和IL-10产生。结论众生丸可通过减轻炎症,调节免疫而减轻H1N1亚型流感病毒所致小鼠肺部病变,提高染毒小鼠存活率。Objective To study the protection and Immunomodulatory mechanism of Zhongsheng Pill on mice infected with H1N1 influenza virus. Methods The model of viral pneumonia was established by dripping H1N1 virus nasally in mice,with Tamiflu as the positive drug. The lung index,histologic and morphologic changes of lungs,survival rate,the levels of T lymphocyte subpopulation,Interferon-γ（ IFN-γ） and interleukin-10（ IL-10） in mice model were investigated. Results Compared with the control group,the model group treated with Zhongsheng Pill was shown a reduced level of lung index,increased survival rate,regulated level of T lymphocyte subpopulation,induced production of IFN-γ and IL-10. Conclusion Zhongsheng Pill can improve the survival rate through relieving the inflammation and regulating the immune function to reduce the lung lesions caused by H1N1 influenza virus.

关 键 词：众生丸 H1N1亚型流感病毒 炎症 免疫 细胞因子 

分 类 号：R285.5[医药卫生—中药学]