不同粒度赤芍药材抗肝纤维化作用的谱效关系研究  

Spectrum-activity relationship on anti-hepatic fibrosis efficacy of Radix Paeoniae Alba with different particle sizes

作　　者：陈红鸽[1] 肖云芝[1,2] 刘朝勇[1,2] 韩晋[1] 袁海龙[1] 

机构地区：[1]中国人民解放军第三〇二医院,北京100039 [2]成都中医药大学药学院

出　　处：《环球中医药》2015年第4期428-431,共4页Global Traditional Chinese Medicine

基　　金：国家重大科研专项新药创制重大专项(2011ZX09201-201-14);国家自然科学基金(81073069)

摘　　要：目的研究不同粉碎粒度赤芍药材的高效液相指纹图谱与其抗纤维化作用的谱效关系,揭示赤芍药材抗肝纤维化作用的"药效成分组"。方法对赤芍药材进行不同程度的粉碎,用高效液相色谱法测定各粉碎粒度赤芍提取液的指纹图谱,确定特征共有峰。以肝星状细胞LX-2为模型研究其对肝纤细胞的抑制效果,采用SPSS软件对数据进行相关分析研究谱效相关性。结果 300目与200目赤芍提取液对肝星状细胞LX-2的抑制效果无差异(P>0.05),但与100目、150目赤芍提取液抑制效果比较具有显著性差异(P<0.01)。各粉碎粒度赤芍药材高效液相指纹图谱中共有19个特征共有峰,其中峰16、峰9与肝星状细胞LX-2抑制效果(OD值)的相关性分别呈极弱相关、弱相关,峰7、峰17呈强相关,其余各峰均呈极强相关。结论 200目为赤芍药材较优粉碎粒度。Objective To investigate the spectrum-activity relationship between HPLC fingerprints of Radix Paeoniae Alba with different particle sizes and anti-hepatic fibrosis efficacy and to explore the＂active components group＂for anti-hepatic fibrosis efficacy of Radix Paeoniae Rubra. Methods Radix Paeoniae Alba with different particle sizes were determined by the HPLC method. The representative standard fingerprint was calculated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（ Version 2004A）. The LX-2 hepatic stellate cells was used as a hepatic fibrosis model to study the inhibitory effect of Radix Paeoniae Alba on hepatic fibrosis cells. The spectrum-effect relationship was established by SPSS 19. 0 software. Results Compared with 300 mesh,200 mesh showed no significant difference（ P〉0. 05）,but 100 mesh and 150 mesh exhibited significant difference（ P〈0. 01） in the inhibition effect of Radix Paeoniae Alba on hepatic fibrosis cells. 19 common peaks in the HPLC-fingerprint of Radix Paeoniae Alba were obtained. It was tentatively concluded that peak 16,peak 9showed a very weak correlation,weak correlation with LX-2 cells inhibitory effect-OD value in 19 characteristic peaks. Peak 7 and peak 17 have better correlation. Other peaks have the strongest correlation. Conclusion 200 mesh is the optimum particle size for Radix Paeoniae Rubra.

关 键 词：赤芍 指纹图谱 抗肝纤维化作用 谱效关系 粒度 

分 类 号：R285.5[医药卫生—中药学]