HPLC和TLC法对蓝色签字笔墨水分类鉴定研究  

Study on the Classifi cation of Blue Gel Pen inks by high performance liquid chromatography and thin layer chromatography

作　　者：陈明星[1] 苏佳利[2] 潘伟[1] 邹洪[3] 

机构地区：[1]北京大学化学与分子工程学院,北京100871 [2]中国科学院大学化学与化工学院,北京100043 [3]首都师范大学化学系,北京100048

出　　处：《现代仪器与医疗》2015年第4期61-64,共4页Modern Instruments & Medical Treatment

基　　金：北京市教育委员会基金项目(No.KM201110028009)

摘　　要：结合薄层色谱法(TLC)和高效液相色谱法(HPLC),分析市售32种不同厂家、不同品牌以及同一品牌不同规格的蓝色签字笔油墨成分。首先采用正丁醇:无水乙醇:水:冰醋酸=6:2:2:1(体积比)为展开剂,用TLC法对32支不同产地不同牌号蓝色签字笔字痕分析,再以甲醇-NH4HCO3缓冲盐体系为流动相,流速0.8m L/min进行高效液相色谱分析。根据色谱峰个数,可将32种蓝色签字笔分成9类。Thin layer chromatography （TLC） and high performance liquid chromatography （HPLC） were employed to analyze the components contained in 32 different blue gel pen inks in this study. The components of 32 blue gel pen inks were analyzed by TLC with developing solvent containing n-butanol： ethanol： water： glacial acetic acid=6： 2： 2： 1. A better separation was archived using HPLC with mobile phases containing ammonium acid carbonate. The two methods are complementary to each other. This analysis of blue gel pen inks was found to be effective and credible.

关 键 词：高效液相色谱 薄层色谱 蓝色签字笔 分类 

分 类 号：O657[理学—分析化学]