抵抗素及葡萄糖转运蛋白-1在妊娠期糖尿病患者胎盘滋养层细胞中的表达及意义  

Expression of Resistin and Glucose Transporter-1 in Human Term Placenta Tissue of Gestational Diabetes Mellitus

作　　者：于勇[1] 熊国平[2] 丁娟[1] 汪琳[1] 

机构地区：[1]武汉大学基础医学院组织学与胚胎学教研室,湖北武汉430071 [2]武汉市中心医院妇产科,湖北武汉430014

出　　处：《武汉大学学报（医学版）》2015年第4期520-523,共4页Medical Journal of Wuhan University

摘　　要：目的:检测抵抗素及葡萄糖转运蛋白(GLUT)-1在正常及妊娠期糖尿病患者胎盘滋养层细胞中的表达,探讨其差异表达的临床意义。方法:取足月妊娠的正常及妊娠期糖尿病患者的胎盘组织,分为对照组(正常组)和实验组(妊娠糖尿病组),常规固定、石蜡包埋、切片、HE染色观察两组滋养层细胞的形态学变化;免疫组织化学SP法检测抵抗素及GLUT-1在两组滋养层细胞中的表达;图像分析技术计量胎盘滋养层细胞中免疫组化阳性结果的平均光密度值。结果:两组胎盘组织的HE染色切片未见明显形态学的改变;免疫组织化学结果显示:抵抗素在妊娠糖尿病组胎盘滋养层细胞中表达(44 960.70±4 077.40)较正常组(26 683.02±2 421.22)明显增高,差异具有显著性(P<0.01);GLUT-1在妊娠糖尿病组胎盘滋养层细胞中的表达(63 817.00±24 160.45)较正常组(110 460±11 395.80)明显降低,差异具有显著性(P<0.01)。结论:抵抗素可能通过下调胎盘滋养层细胞中GLUT-1的表达,影响母胎之间的葡萄糖的转运,进而影响胎儿的生长发育。Objective： To explore the expression of resistin and glucose transporter-1 （GLUT-1）in pla- cental trophoblast cells of normal pregnancy and gestational diabetes mellitus（GDM）. Methods： The human term placenta tissue from normal pregnancies and gestational diabetes mellitus were divided into control and GDM groups. The morphological changes of trophoblastic cells were ob- served by using light microscope. Immunohistochemical technology was employed to detect the expression of resistin and GLUT-1 in trophoblastic cells. Results： There was no obviously mor- phological difference between two groups under the light microscope; Immunohistochemical re- sult showed that the expression of resistin was significantly higher in gestational diabetes mellitus group than those in normal group （P〈0.01）. The expression of GLUT-1 was significantly lowerthan those in gestational diabetes mellitus group （P〈0.01）. Conclusion. Resistin may impact the transport of glucose between mother and fetus through down-regulating the expression of GLUT- 1, and thus affect development of fetus.

关 键 词：抵抗素 葡萄糖转运蛋白-1 妊娠期糖尿病 滋养层细胞 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]