全反式视黄醛影响内异症内膜基质细胞迁移及视黄醇脱氢酶10 mRNA表达  

Effect of All-Trans-Retinal on the Migration of Endometrial Stromal Cells and Expression of RDH10 mRNA in Endometriosis

作　　者：李艳敏[1,2] 张昌军[2] 董毅飞[2] 邓锴[2] 彭海英[2] 刁红录[2] 

机构地区：[1]武汉大学中南医院,湖北武汉430071 [2]湖北医药学院附属人民医院生殖医学中心,湖北十堰442000

出　　处：《武汉大学学报（医学版）》2015年第4期524-528,共5页Medical Journal of Wuhan University

基　　金：湖北省教育厅科学计划项目资助(编号:B20112103;D20142101;Q20122402)

摘　　要：目的:探讨全反式视黄醛(at-Ral)对体外培养人子宫内膜异位症(EMT)在位内膜基质细胞迁移和视黄醇脱氢酶(RDH)10mRNA表达的影响。方法:体外分离培养EMT患者在位子宫内膜基质细胞(EMT-ESC)及正常人子宫内膜基质细胞(N-ESC),采用细胞划痕运动分析及real time PCR方法分别观察不同浓度at-Ral对细胞迁移及RDH10mRNA表达情况。结果:N-ESC的迁移距离及RDH10的表达随at-Ral浓度的升高而减少,1,10μmol/L组与对照组比较差异有统计学意义(P<0.05),而EMT-ESC的迁移距离及RDH10的表达随at-Ral浓度的升高而增加,1,10μmol/L组与对照组比较差异有统计学意义(P<0.05)。结论:at-Ral对内异症患者在位子宫内膜基质细胞的迁移及RDH 10mRNA表达有促进作用。Objective. To investigate the effect of all-trans-retinal（at-Ral）on the migration and retinol dehydrogenase （RDH10） mRNA expression of in vitro cultured human endometrial eutopic endo- metrial stromal cells. Methods. Endometrial stromal cells were isolated in vitro in normal women （N-ESC） and women with endometriosis （EMT-ESC）. Then we observed the effects of different concentrations of at-Ral by cell scratch assay and real time PCR. Results. The migration distance and expression of RDH 10 mRNA of N-ESC decreased with the increased concentration of at-Ral. There were significant differences between 1, 10 μmol/L groups and the control group （P〈0.05）, while the migration distance and expression of RDH 10 mRNA of EMT-ESC increased with the increased concentration of at-Ral. There were significant differences between 1, 10μmol/L groups and the control group （P〈0.05）. Conclusion： At-Ral can promote migration and expression of RDH10 mRNA of eutopic endometrial stromal cell of endometriosis.

关 键 词：视黄醛 迁移 细胞 子宫内膜异位症 生殖 

分 类 号：R711.71[医药卫生—妇产科学]