LPS对绵羊输卵管上皮细胞β-防御素-1表达的影响及其机制研究  

Study on the beta-defensin 1 expression in ovine oviduct epithelial cells induced by lipopolysaccharide

作　　者：李琦[1] 曹贵方[1] 智达夫[1] 任丽欣 延沁 刘默宁 郑欣欣[1] 

机构地区：[1]内蒙古农业大学兽医学院动物组织胚胎与发育生物学研究室,内蒙古呼和浩特010018

出　　处：《中国预防兽医学报》2015年第6期477-480,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金项目(31360593);国家自然科学基金项目(31060328)

摘　　要：为研究脂多糖(LPS)对绵羊输卵管上皮细胞β-防御素-1(SBD-1)表达的影响及其分子机制,本研究通过荧光定量PCR检测不同浓度LPS作用不同时间对绵羊输卵管上皮细胞SBD-1 m RNA的相对表达量的影响,通过western blot检测经LPS处理后P38 MAPK通路的活化情况,通过荧光定量PCR检测经P38 MAPK通路抑制剂(SB203580和SB202190)处理后LPS对SBD-1 m RNA相对表达量的影响。结果表明,LPS呈浓度和时间依赖方式诱导绵羊输卵管上皮细胞中SBD-1的表达,100 ng/m L的LPS在12 h诱导效果最佳。进一步研究显示,100 ng/m L的LPS可以显著激活P38 MAPK通路,而其抑制剂SB203580和SB202190可以阻断LPS对SBD-1的诱导作用。这些结果表明,LPS可以诱导绵羊输卵管上皮细胞表达SBD-1,并且P38 MAPK参与调控这个过程。本实验结果为进一步研究β-防御素的调控机制,探索输卵管炎发病机理以及合理开发输卵管炎相关药物提供了实验依据。To explore the effect of lipopolysaccharide （LPS） on the expression of [3-defensin 1 （SBD-1） in ovine oviduct epithelial cells, the ovine oviduct epithelial cells cultured in vitro were treated with LPS at doses from 10 ng/mL to 1 mg/mL, respectively, and the total RNA were extracted from the cells at 0 h to 24 h for examinations by real-time RT-PCR. The results showed that LPS was able to induce the expression of SBD-1 in a timeand concentration- dependent manner. Meanwhile, the P38 MAPK signaling was activated by LPS at 20 min. Moreover, the treatment with SB203580 and SB202190 （inhibitors of P38 MAPK signaling） were able to markedly inhibit the mRNA expression of SBD-1, which was induced by 100 ng/mL of LPS. In conclusion, the results indicated that LPS might function through P38 MAPK signaling to induce the expression of SBD-1 in the ovine oviduct epithelial cells. We believe that these observations have broad physiological and clinical implications for a better understanding of ovine oviduct physiology and pathology.

关 键 词：脂多糖 β-防御素-1 P38 MAPK 

分 类 号：S852.6[农业科学—基础兽医学]