广州地区汉族人群血小板抗原基因多态性及频率分布  

Gene frequencies and genetic polymorphism of human platelet antigen in Guangzhou Han population

作　　者：陈扬凯[1] 叶欣[1] 夏文杰[1] 丁浩强[1] 徐秀章[1] 邓晶[1] 邵媛[1] 王嘉励[1] 付涌水[1] 

机构地区：[1]广州血液中心临床输血研究所,510095

出　　处：《国际输血及血液学杂志》2015年第3期207-211,共5页International Journal of Blood Transfusion and Hematology

基　　金：广州市科技计划应用基础研究计划项目资助（2010Y1-C471）;广州市医药卫生科技重点项目资助（20121A021020）

摘　　要：目的 探讨广州地区汉族人群人类血小板抗原（HPA）-7Hita及HPA-12,-18,-19,-20,-21等位基因多态性及其频率分布情况.方法 选取2010年9月至2011年9月于广州血液中心参与志愿无偿献血,且符合《献血者健康要求（GB18467-2001）》的广州地区汉族献血者中,采用系统随机抽样方法随机选择95例献血者作为研究对象.采集并留取献血者血液标本;分别采用序列特异性引物-聚合酶链反应（PCR-SSP）及序列特异性引物-多重聚合酶链反应（multi-PCR-SSP）对研究对象血液标本进行HPA-7Hita及HPA-12,-18,-19,-20,-21等位基因分型检测,并对广州地区汉族人群HPA-7Hita及HPA-12bw、-18bw、-19bw、-20bw、-21bw等位基因频率与不同国家、地区人群进行比较.结果 ①95例广州汉族志愿无偿献血者均为HPA-7a、-12a、-18a、-19a、-20a、-21a,等位基因频率均为100％,均未发现HPA-7Hita及HPA-12bw、-18bw、-19bw、-20bw、-21bw等位基因;②进一步分析调查结果显示,广州地区汉族人群中HPA-7Hita等位基因频率低于日本人群,且差异有统计学意义（P＜0.05）,HPA-12bw等位基因频率亦低于德国人群,且差异有统计学意义（P＜0.05）;HPA-18bw、-19bw、-20bw、-21bw等位基因频率则分别与法国、巴西、中国、日本的报道相符.结论 本研究初步获得广州地区汉族人群HPA-7Hita及HPA-12,-18,-19,-20,-21等位基因频率分布情况,为临床相关疾病的诊治及预测提供一定实验数据基础及检测方法.Objective To investigate the gene frequencies of human platelet antigen （HPA）-7Hita and HPA-12,-18,-19,-20,-21 in Guangzhou Han population.Methods A total of 95 blood donors were randomly selected into this study from voluntary blood donors in Guangzhou from September 2010 to September 2011,via systematic random sampling method.All these blood donors were health and qualified.Blood samples of the 95 blood donors were collected.Polymerase chain reaction-sequence specific primer （PCR-SSP） and multiple polymerase chain reaction-sequence specific primer （multi-PCR-SSP） were used to detected genotypes of HPA-7Hita,-12,-18,-19,-20,-21.Gene frequencies of HPA-7Hita and HPA-12bw、-18bw、-19bw、-20bw、-21bw were compared among different countries and areas.Results ① For these 95 blood donors in Guangzhou Han population,gene frequencies of HPA-7a、-12a、-18a、-19a、-20a、-21a were all 100％.No HPA-7Hita and HPA-12bw、-18bw、-19bw、-20bw、-21bw allele were found.②Further investigation revealed that gene frequency of HPA-7Hita in Guangzhou Han population was significantly lower than that in Japan population（P＜0.05）.Gene frequency of HPA-12bw was also significantly lower than that in German population（P＜0.05）.Gene frequencies of HPA-18bw,-19bw,-20bw,-21bw were the same as these in France,Brazil,China and Japan.Conclusions This study gained preliminary datas on gene frequencies of HPA-7Hita and HPA-12,-18,-19,-20,-21 in Guangzhou Han population,and provide experimental data and detection method for diagnosis,treatment and prognosis of clinical related diseases.

关 键 词：抗原 人血小板 基因频率 多态性 单核苷酸 

分 类 号：R446.6[医药卫生—诊断学]