重型颅脑创伤大鼠后期脑水肿研究及牛磺酸转运体的作用  

Protection role of taurine transporter in rats brain edema followed severe traumatic head injury

作　　者：蔡英[1] 黄慧玲[1] 范维佳[1] 武俏丽[1] 李晓茜[1] 苏彦华[1] 温晓昶[1] 

机构地区：[1]天津市环湖医院,天津市神经外科研究所,天津市脑血管与神经变性重点实验室,300060

出　　处：《天津医药》2015年第6期607-611,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(30973089);天津市应用基础及前沿技术研究计划项目(10JCYBJC23200);天津市卫生局科技基金项目(2012KR09)

摘　　要：目的研究牛磺酸转运体(Tau T)在牛磺酸调节重型颅脑创伤大鼠后期脑水肿中的作用。方法将40只大鼠按随机数字表法分为4组:假手术组(Sham组)、脑外伤组(TBI组)、牛磺酸预防组(Pre-Tau组)和牛磺酸治疗组(Tau组)。液压冲击法制作重型颅脑创伤大鼠模型。Pre-Tau组和Tau组分别在造模前或后给予120 g/L的牛磺酸溶液,其他两组给予等量的等渗生理盐水。7 d后用干湿重法检测脑组织中脑水含量,实时荧光定量PCR和West-ern Blot方法检测Tau T和水通道蛋白(AQP4)的m RNA表达及蛋白含量。结果与Sham组相比,TBI组脑水含量、AQP4的m RNA和蛋白表达水平均显著升高,Tau T的m RNA和蛋白表达水平显著降低。与TBI组比较,Pre-Tau组和Tau组脑水含量、AQP4的m RNA和蛋白表达水平显著降低、Tau T的m RNA表达明显升高;Tau组Tau T的蛋白表达明显升高;Pre-Tau组Tau T的蛋白表达有所升高,但差异无统计学意义。AQP4的m RNA和蛋白表达水平与脑水含量呈正相关(r分别为0.621和0.457),AQP4的m RNA与蛋白表达亦呈正相关(r=0.459)。结论牛磺酸通过提高重型颅脑创伤大鼠后期Tau T的表达水平,降低脑水含量和AQP4的表达,发挥神经元保护作用。Objective To investigate the effect of taurine transporter in the process of protection of brain edema in rats with severe traumatic head injury. Methods A total of 24 Male Sprague-Dawley rats were randomly divided into 4 groups. Except the control rats （Group Sham）, all other three groups were subjected to lateral fluid percussion head injury. The TBI （Traumatic brain injury） models （Group TBI） and surgical control rats （Group Sham） were injected with saline through caudal vein after surgery, while the Taurine prevention and Taurine treatment models （Group Pre Tau and Group Tau） were injected with 120 g/L taurine solution before or after surgeries respectively. Water content in each brain, mRNA and protein expres-sion of aquaporin 4 and taurine transporter in the injured rat brain hemispheres were all evaluated over the time course of the study （7 d） in each group. Results Compared with rats in Group Sham, water content in each brain increase, mRNA tran-scription and protein expression of AQP4 were both up regulated but the mRNA transcription and protein expression of TauT were both down-regulated in rats in TBI group. Compared with rats in TBI group, brain water content, mRNA transcription and protein expression of AQP4 all decrease while mRNA transcription and protein expression of TauT all increase in rats in Pre tau and Tau groups. There is no statistical difference of TauT expression between rats in pre-tau group and Tau group. Conclusion Taurine exert its neuron protection role through draining water content from brain and down regulating expres-sion of AQP4 but rising expression of TauT after TBI.

关 键 词：牛磺酸 脑损伤 膜转运蛋白质类 脑水肿 水通道蛋白质4 大鼠 SPRAGUE-DAWLEY 牛磺酸转运体 

分 类 号：R651.15[医药卫生—外科学]