黄连素通过上调Smad7减轻2型糖尿病大鼠肾脏损伤  

Renoprotective Effect of Berberineon Kidney Injury of Type 2 Diabetic Rat Modelvia Upregulating Smad7

作　　者：孙斯凡[1,2] 张浩军[2] 赵婷婷[2] 严美花[2] 李平[2] 

机构地区：[1]中国医学科学院北京协和医学院研究生院,北京100730 [2]中日友好医院临床医学研究所,免疫炎性疾病北京市重点实验室,北京100029

出　　处：《中国中西医结合肾病杂志》2015年第5期392-395,I0002,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：科技部国际科技合作项目(No.2011DFA31860);国家自然科学基金资助项目(No.81173422;81130066)

摘　　要：目的:观察黄连素对2型糖尿病大鼠肾脏损伤的保护作用,并探讨可能机制。方法:将大鼠分为正常对照组、糖尿病模型组以及黄连素治疗组,给药20周后检测血糖、24 h尿蛋白,观察肾脏病理改变,并检测炎症(IκBα)及纤维化(TGF-β1、TβRI、Smad7)信号通路。结果:与正常对照组比较,糖尿病大鼠肾重指数增加,24 h蛋白尿维持在高水平,肾脏病理显示肾小球系膜基质增生,肾小管扩张变性且排列紊乱,小管间质存在炎症浸润;黄连素治疗显著降低模型大鼠肾重指数及24 h蛋白尿,减少肾皮质TGF-β1、TβRI表达,上调Smad7和IκBα表达。结论:黄连素对2型糖尿病大鼠具有肾脏保护作用,机制可能是通过上调Smad7表达从而改善肾脏炎症及纤维化。Objective： To observe the renoprotective effect and mechanisms of berberine on a type 2 diabetes rat model.Methods： Wistar rats were randomly divided into normal control group,diabetic group and berberine- treated group. Rats were sacrificed 20 weeks later. We detected the kidney weight index,24 h urine protein,morphology study,inflammationand fibrosis markers,such as IκBα,TGF- β1,TβRI and Smad7. Results： Compared with normal rats,the diabetic rats showed higher kidney weight indexand 24 h urine protein,severe morphological changes; berberine treatment decreased kidney weight index and 24 h urine protein,alleviatedmorphological changes,suppressed the expression of TGF- β1,TβRI,meanwhile increased the expression of IκBα and Smad7. Conclusion： Berberine has renoprotectiveeffect on a type 2 diabetic rat model. The mechanism might suppressinflammation and fibrosis via upregulating expression of Smad7.

关 键 词：黄连素 糖尿病肾病 SMAD7 IΚBΑ TΒRI 

分 类 号：R587.2[医药卫生—内分泌]