慢性乙型肝炎患者外周血e抗原滴度及病毒载量与T细胞亚群分布的关系  

Relations between HBeAg titer and viral loads with T lymphocyte subsets distribution in the peripheral blood of chronic hepatitis B patients

作　　者：吴敏娟[1] 胥萍[2] 陈慧[2] 徐俊驰[2] 李明[3] 马亚娜[1] 

机构地区：[1]苏州大学医学部公共卫生学院,江苏省苏州市215123 [2]江苏省苏州市第五人民医院检验中心,215007 [3]江苏省苏州市第五人民医院传染科病区,215007

出　　处：《国际流行病学传染病学杂志》2015年第3期157-161,共5页International Journal of Epidemiology and Infectious Disease

基　　金：江苏省卫生厅资助项目（KF200974）;苏州市科学技术局科技计划项目（SYS201433、SZS201006）

摘　　要：目的分析慢性乙型肝炎患者HBeAg滴度及HBVDNA载量与T细胞亚群分布的关系。方法选取苏州市第五人民医院2013年1-8月住院乙型肝炎患者87例为研究对象，50例健康体检者为正常对照。采用化学发光微粒子酶免疫法测定患者外周血血清中HBeAg滴度：采用实时荧光定量PCR法检测外周血HBVDNA；采用流式细胞术测定患者和对照外周血T细胞亚群数量。87例乙型肝炎患者，HBeAg阳性54例，阴性33例；HBVDNA阳性60例，阴性27例。54例HBeAg阳性患者根据HBeAg含量分成3组：HBeAg低滴度组、中滴度组和高滴度组。60例HBVDNA阳性患者根据病毒载量分成3组：HBVDNA低载量组、中载量组和高载量组。结果HBeAg阳性组的CD3＋和CD4＋T细胞所占百分比分别为（60．36±6．62）％和（29．26±7．62）％，均低于对照组（t=8．95、7．19，P均〈0．05）；CD8叩细胞所占百分比为（26．17±6．34）％，高于对照组的（23．70±4．53）％（t=-2．27，P〈0．05）。不同HBVDNA载量组与正常对照组之间CD3＋、CD4＋和CD8＋T细胞百分比的差异均有统计学意义（F=2．93、3．97和7．67，P均〈0．05）。HBeAg阴性、HBeAg阳性与正常对照组之间CD3＋、CD4＋、CD8＋T细胞所占百分比的差异均有统计学意义（F=30．68、22．86和3．31，P均〈0．05）。结论乙型肝炎患者的外周血HBeAg滴度及HBVDNA载量不同，其T细胞亚群分布也不同。测定T细胞亚群或可了解患者的免疫状态，为及时调节患者的免疫功能和抗病毒治疗的临床应用提供依据。Objective To analyze the relations between the HBeAg titer and viral loads with T lymphocyte subsets distribution in chronic hepatitis B patients. Methods There were 87 CHB patients in the Fifth People＇s Hospital of Suzhou from January to August in 2013 as research objects and 50 healthy physical examinations as normal group. HBeAg was detected by chemiluminescence method and the serum HBV viral loads were detected by the real2 time quantitative PCR. The peripheral T-lymphocyte subsets were detected by flow eytometry. In 87 hepatitis B patients, there were 54 cases of HBeAg positive and 33 cases of negative; and there were 60 cases of HBV DNA positive and 27 cases of nagetive. Fifty-four HBeAg positive cases were divided into 3 groups by HBeAg content： low titer group, middle titer group and high titer group. Sixty HBV DNA positive cases were divided into 3 groups by viral load： high HBV DNA load group, middle load group and low load group. Results The percentages of CD3＋ and CD4＋ T cells in HBeAg positive group were （60.36±6.62）% and （29.26±7.62）%, and were both lower than control group（t= 8.95, 7.19, Pboth〈0.05）. The percentage of CD8＋T cells were （26.17±6.34）%, and were higher than the control group （t=-2.27, P〈0.05）. The differences of CD3＋, CD4＋ and CD8＋T cells among different HBV DNA load group and control group had statistical significances （F=2.93, 3.97 and 7.67, P all〈0.05）. The differences of CD3＋, CD4＋ and CD8＋ T cells among HBeAg positive group, negative group and control group had statistical significances （F=30.68, 22.86 and 3.31, P all 〈0.05）. Conclusions The relations between HBeAg titer and viral load with T lymphocyte subsets in chronic hepatitis B patients are very close, so detecting the T lymphocyte subsets can be conductive to understand the patient＇s immune function and provide the basis for the clinical to regulate the immune function of the patients promptly and apply antiviral therapy.

关 键 词：肝炎病毒 乙型 肝炎e抗原 乙型 病毒载量 T细胞 

分 类 号：R512.62[医药卫生—内科学]