术前应用乌司他丁对小鼠肝大部分切除术后肝功能及一磷酸腺苷活化蛋白激酶信号通路的影响  

Preoperative ulinastatin promotes liver function via adenosine monophosphateactivated protein kinase signaling pathway in mice after partial hepatectomy

作　　者：訾雪剑 姚辉[2] 伏晓[2] 仇毓东[1,2] 贾文俊[2] 

机构地区：[1]南京医科大学鼓楼临床医学院,江苏省南京市210008 [2]南京大学医学院附属鼓楼医院,江苏省南京市210008

出　　处：《世界华人消化杂志》2015年第15期2363-2372,共10页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81470866~~

摘　　要：目的:探讨术前应用乌司他丁对小鼠70%肝切除术后肝功能及一磷酸腺苷活化蛋白激酶(adenosine monophosphate-activated protein kinase,AMPK)信号通路的影响.方法:♂ICR小鼠48只,体质量25-30 g,随机均分为70%肝切除对照组和乌司他丁组.检测术后第1、3、5、7天肝功能指标,残肝组织病理学改变以及AMPK蛋白活化情况.结果:与对照组比较,乌司他丁组术后第1、3天谷草转氨酶水平较低,差异有统计学意义(P<0.05);与对照组比较,乌司他丁组术后第3天乳酸脱氢酶水平有所降低,差异有统计学意义(P<0.05).与对照组比较,Western blot结果显示乌司他丁组肝切除术后早期AMPK磷酸化较晚;与对照组比较,Western blot结果显示乌司他丁组肝切除术后胆盐输出泵蛋白(bile salt export protein,BSEP)水平较高.结论:术前应用乌司他丁可以保护术后肝功能,降低肝损伤,促进术后肝功能的恢复.其机制可能与维持肝细胞能量供应以及促进肝细胞极化有关.AIM：To investigate the effect of preoperative ulinastatin on liver function and adenosine monophosphate-activated protein kinase（AMPK） signaling pathway in mice after 70%hepatectomy.METHODS：A total of 48 male ICR mice weighing 25-30 g were randomly divided into a control group and an ulinastatin group.Blood and liver tissue samples were collected on days 1,3,5 and 7 after 70%hepatectomy.Serum alanine aminotransferase（ALT）,aspartate transaminase（AST）,total bilirubin（TB） and lactate dehydrogenase（LDH） levels were measured,while liver tissues were used for morphology study and AMPK signaling analysis.RESULTS：Serum AST levels on days 1and 3 and serum LDH levels on day 3 after hepatectomy were significantly lower in the ulinastatin group than in the control group（P 〈 0.05）.Subsequent mechanism analysis revealed that AMPK signaling pathway was activated significantly later and bile salt export protein（BSEP） was expressed significantly higher in the ulinastatin group than in the control group.CONCLUSION：Ulinastatin can reduce liver injury by promoting the recovery of liver function after liver resection,via mechanisms associated with AMPK signaling.

关 键 词：肝切除 乌司他丁 肝功能 一磷酸腺苷活化蛋白激酶信号通路 

分 类 号：R657.3[医药卫生—外科学]