安胃汤影响大鼠胃溃疡恶性病变组织中P21ras和P53蛋白表达  

Effect of Anwei decoction on expression of P21ras and P53 in malignant ulcers in rats

作　　者：吴德坤[1] 黄瑞诚[2] 郑景辉[1] 唐友明[1] 莫启章[1] 秦伟夫[1] 

机构地区：[1]广西中医药大学附属瑞康医院,广西壮族自治区南宁市530011 [2]广西中医药大学继续教育学院,广西壮族自治区南宁市530001

出　　处：《世界华人消化杂志》2015年第15期2420-2424,共5页World Chinese Journal of Digestology

基　　金：广西自然科学基金面上资助项目;No.2014GXNSFAA118140~~

摘　　要：目的:探究中药剂安胃汤对实验制作的胃溃疡恶性病变组织大鼠模型的P21ras和P53蛋白表达产生的影响.方法:本实验将72只大鼠按随机原则分成空白组、胃溃疡模型组、治疗组(分大、中、小剂量组)、对照组(胃复春),模型制作采用胃黏膜表面局部投予乙酸方法,完成大鼠胃溃疡模型,免疫组织化学(SP)法检测P21ras和P53蛋白表达.结果:安胃汤治疗后的各组和胃复春组治疗后与病理组相比,溃疡组织的P21ras表达量均明显增加,差异存在统计学意义(P<0.05);安胃汤中剂量组的溃疡组织中P21ras表达量与胃复春组治疗后相比差异存在统计学意义(P<0.05);安胃汤中剂量与大剂量组溃疡组织中,P53表达与病理组比较差异存在统计学意义(P<0.05);安胃汤组的胃部溃疡面均出现明显愈合现象.结论:安胃汤对大鼠溃疡恶性病变组织中P21ras和P53表达有明显的调节作用,可增强胃部溃疡组织的修复效率,可能是安胃汤治疗胃溃疡恶性病变的主要机制之一.AIM：To investigate the effect of Anwei decoction on P21 ras and P53 protein expression in experimental malignant ulcers in a rat model.METHODS：Seventy-two rats were randomly divided into a blank control group,a model control groups,high-,medium- and low-dose Anwei decoction group,and a Weifuchun group.Malignant ulcers were induced in rats by applying acetic acid to gastric mucosal surface.Immunohistochemistry was used for detection of P21 ras and P53 protein expression.RESULTS：Compared with the model control group,P21 ras expression levels were significantly decreased in the Anwei decoction groups and Weifuchun group（P 〈 0.05）.P21 ras expression levels in ulcer tissue differed significantly between the medium Anwei decoction group and the Weifuchun group（P 〈 0.05）.Compared with the model control group,P53 expression levels were significantly increased in the high-dose Anwei decoction group and Weifuchun group（P 〈 0.05）.Obvious ulcer healing was noted in Anwei decoction groups.CONCLUSION：Anwei decoction has a significant effect on the expression of P21 ras and P53 in malignant ulcers in rats,and this may be one of mechanisms underlying the therapeutic effect of Anwei decoction against canceration of malignant ulcers.

关 键 词：安胃汤 胃溃疡 P21RAS P53 

分 类 号：R285.5[医药卫生—中药学]