早期香烟烟雾暴露对哮喘小鼠 tDC、Treg数量和功能的影响  

Effects of exposure to cigarette smoke on the counts and functions of tDC and Treg cells in a mouse ;model of asthma

作　　者：黄飞[1] 尹嘉宁[2] 尤海龙[2] 李亚男[2] 

机构地区：[1]吉林大学中日联谊医院骨科,长春130033 [2]吉林大学第一医院小儿呼吸一科,长春130021

出　　处：《中华微生物学和免疫学杂志》2015年第5期353-358,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（81200013）;吉林大学白求恩B计划（2014202）

摘　　要：目的：在整体水平研究香烟烟雾早期暴露对哮喘小鼠调节性T细胞和耐受型DC数量和功能的影响。方法幼鼠香烟烟雾暴露3周，进入成年后建立卵清蛋白（ OVA）致敏的BALB／c小鼠依随机表哮喘模型。40只BALB／c小鼠依随机表随机分为4组：生理盐水／空气组、生理盐水／香烟烟雾组、OVA／空气组和OVA／香烟烟雾组，每组10只。通过流式细胞术检测各组小鼠外周血单个核细胞中Treg百分比，实时定量 PCR方法检测肺组织中 Foxp3 mRNA 表达；分离肺脏树突状细胞（ DC），用流式细胞仪分析DC表面共刺激分子CD80和CD86表达的变化；并比较各组小鼠肺泡灌洗液中IFN-γ、IL-4、IL-6、IL-10、IL-12p40、IL-17A和TGF-β1的含量差异。结果幼年时期香烟烟雾暴露可显著增加哮喘小鼠外周血单个核细胞中Treg百分比和Foxp3 mRNA表达，而且可增加正常小鼠和哮喘小鼠支气管肺泡灌洗液（ BALF）中TGF-β1、IL-10的含量；幼年时期香烟烟雾暴露可显著减弱哮喘小鼠DC细胞表面分子CD80、CD86表达强度，而且可降低哮喘小鼠BALF中IL-12p40、IL-6的含量；幼年时期香烟烟雾暴露可显著增加哮喘小鼠BALF中IFN-γ、IL-4、IL-17A的含量。结论幼鼠时期香烟烟雾暴露参与哮喘小鼠的细胞免疫功能紊乱，这可能为哮喘的防治提供新思路。Objective To investigate the effects of exposure to cigarette smoke on the counts and functions of tolerogenic dendritic cells（tDC） and regulatory T cells（Treg） in a mouse model of asthma. Methods Forty female BALB/c mice at age 20 days were randomly allocated into four groups including the normal saline （ NS）/air treatment group, the NS/cigarette smoke （ CS） treatment group, the ovalbumin （OVA）/air treatment group and the OVA/CS treatment group （n=10）.A mouse model of asthma was es-tablished by intraperitoneally injecting OVA in an interval of two weeks to cigarette smoke for three weeks. The percentages of Treg cells in PBMCs and the changes of CD80 and CD86 expression on the surface of DCs of the mice in each group were analyzed by flow cytometry analysis.The transcriptional levels of forkhead box P3 （ Foxp3） in lung tissues were measured by real-time quantitative PCR.The levels of IFN-γ, IL-4, IL-6, IL-10, IL-12p40, IL-17A and TGF-β1 in bronchoalveolar lavage fluid （ BALF） samples were measured by ELISA.Results Exposing young mice to cigarette smoke significantly increased the percentages of Treg cells in PBMCs and the expression of Foxp3 at mRNA level in lung tissues of mice with asthma.High levels of TGF-β1 and IL-10 were detected in BALF samples of the heathy mice and those with asthma exposed to CS at a young age.The mice with asthma exposed to CS at an early age showed decreased expression of CD80 and CD86 on the surface of DCs.Lower levels of IL-12p40 and IL-6 and higher levels of IFN-γ, IL-4 and IL-17A in BALF samples were observed in mice from the OVA/CS treatment group.Conclusion Expo-sing to cigarette smoke at an early age was involved in the dysfunction of T cell-mediated immune responses in mice with asthma.This study might provide new ideas for the prevention and treatment of asthma.

关 键 词：香烟烟雾 调节性T细胞 耐受型DC 

分 类 号：R562.25[医药卫生—呼吸系统]