超高效液相色谱法测定脐橙中橘红2号和苏丹红  

Determination of Citrus Red 2 and Sudan Red in Navelorange by UHPLC

作　　者：鄢兵[1] 张云伟[1] 胡海山[1] 赵淑娥[1] 芦慧[1] 

机构地区：[1]江西省分析测试研究所,南昌330029

出　　处：《理化检验（化学分册）》2015年第6期807-809,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：江西省科技计划项目(20142BBG70032)

摘　　要：采用超高效液相色谱法同时测定脐橙中的橘红2号和苏丹红染料。样品经乙腈超声提取，氨基固相萃取小柱净化后，用Waters ACQUITY UPLC BEH C18色谱柱分离，以乙腈-水为流动相进行梯度洗脱，采用二极管阵列检测器检测，检测波长分别为478nm和515nm。5种染料的质量浓度在0．20~20mg·L叫范围内呈线性，检出F~-（3S／N）在0．31～0．53μg·kg-1之间。加标回收率在87．8％～99．4％之间，测定值的相对标准偏差（n=5）在0．54％～3．1％之间。An UHPLC method was developed for the determnation of dyes of citrus red 2 and sudan red in navelorange. The sample was extracted with acetonitrile and cleaned-up by an amino solid phase extraction column. The analyte was separated on a Waters ACQUITY UPLC BEH C18 column by gradient elution using a mobile phase made up of acetonitrile and water,and detected by a photo-diode array detector at the wavelength of 478 nm and 515 nm. The linearity range of 5 dyes was 0. 20- 20 mg·L- 1,with detection limits in the range of 0. 31- 0. 53 μg·kg- 1. The recovery rates measured by standard addition method were between 87. 8% and 99. 4%,with RSD（ n = 5） were between 0. 54% and 3.11%.

关 键 词：超高效液相色谱法 橘红2号 苏丹红 脐橙 

分 类 号：O652.63[理学—分析化学]