厌氧氨氧化耦合脱氮系统中反硝化微生物研究  

Study on Denitrifying Bacteria in Anaerobic Ammonium-oxidation Coupled with Denitrification System

作　　者：王春香[1] 刘常敬[1] 郑林雪[1] 郑照明[1] 李军[1] 

机构地区：[1]北京工业大学建筑工程学院,北京100124

出　　处：《中国给水排水》2015年第13期19-22,共4页China Water & Wastewater

摘　　要：以厌氧氨氧化耦合异养反硝化脱氮系统作为对象,采用PCR、分子克隆等分子生物学方法,通过构建nir S克隆文库研究了反应器中的反硝化微生物,并进行同源性分析和系统发育树构建。结果表明,从nir S克隆文库中随机挑选的100个克隆子中有60个阳性克隆子,共分为12个OTU,经BLAST比对发现这些微生物主要为变形菌门细菌,其中β-变形菌纲和γ-变形菌纲占据绝对优势,有少部分属于α-变形菌纲。反应器中的优势反硝化菌为β-变形菌纲中的Azoarcus tolulyticus,该菌为苯酚降解菌,对于反应器中苯酚的降解具有重要作用。Denitrifying microorganisms in the anaerobic ammonium oxidation coupled with heterotrophic denitrification system were investigated by using PCR and molecular cloning and building nirS clone libraries. The homology analysis was conducted, and the phylogenetic tree was constructed. 60 positive clones from the 100 clones that selected from the nirS clone libraries were assigned to 12 OTUs. By using BLAST, it was found that these microorganisms were mainly proteobacteria, most of which were β-proteobacteria and γ-proteobacteria, and little of which was α-proteobacteria. The dominant species was Azoarcus tolulyticus, which can decompose phenol in the reactor.

关 键 词：厌氧氨氧化 反硝化菌 PCR nirS克隆文库 

分 类 号：X703[环境科学与工程—环境工程]