活性污泥胞外聚合物在污水处理过程中的荧光特性  

Fluorescence characteristics of extracellular polymeric substances in activated sludge during wastewater treatment processes

作　　者：李志华[1] 刘毅[1] 张芹[1] 张妍[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西北水资源与生态教育部重点实验室,西安710055

出　　处：《环境工程学报》2015年第7期3172-3176,共5页Chinese Journal of Environmental Engineering

基　　金：陕西省科技计划国际合作项目(2013KW2201);博士点基金联合资助课题(20136120110002)

摘　　要：采用三维荧光光谱法对活性污泥的微生物代谢过程进行了分析。通过分析活性污泥进入内源呼吸过程的EPS中荧光性物质的变化规律,发现疏松结合的胞外聚合物(LB-EPS)荧光光谱的微生物代谢产物峰在内源呼吸前期就开始下降,下降比例为11%,而LB-EPS荧光光谱图中的类蛋白荧光峰和紧密结合的胞外物质(TB-EPS)荧光光谱图的类溶解性微生物代谢产物荧光峰在内源呼吸的后期下降,下降比例分别为18%和14%,在前期分别升高了34%和372%。另外,提出了通过疏松的胞外聚合物(LB-EPS)荧光偏移量来分析污水处理过程特性,得出荧光峰位置的偏移量与污水厂的出水COD、氨氮和污泥内源呼吸比具有很好的相关性,相关系数分别为0.943、0.825和0.941。Three-dimension excitation emission matrix（3DEEM） fluorescence spectroscopy was applied to investigate the microbial metabolic process in activated sludge systems.Variation of fluorescence matters in extracellular polymeric substances（EPS） of activated sludge microorganism was studied during the endogenous respiration phase.Results showed that for loosely-bounded EPS（LB-EPS）,peak fluorescence intensity of microbial metabolites decreased by 11% of initial value since early stage of endogenous respiration process,while peak fluorescence intensity of protein-like matter enhanced by 34% in the early stage and then reduced by 18% in the later stage.On the other hand,for tightly-bounded EPS（TB-EPS）,the fluorescence intensity of microbial metabolite peak enhanced by 372% in the early stage of endogenous respiration process and reduced by 14% in the later stage.Additionally,fluorescence peak shift corresponding to LB-EPS was analyzed to characterize the fluorescence properties of activated sludge in biological wastewater treatment process.Results demonstrated that fluorescence peak shift corresponding to LB-EPS showed a high correlation with COD,ammonia nitrogen in the effluent and with endogenous respiration ratio of activated sludge,and the determination coefficients were 0.943,0.825 and 0.941,respectively.

关 键 词：胞外聚合物 三维荧光光谱 出水水质 内源呼吸 

分 类 号：X703[环境科学与工程—环境工程]