脉冲电场对渗透胁迫下玉米幼苗自发光子辐射的影响  

Effect of pulsed electric field on spontaneous photon emission of corn seedlings under osmotic stress

作　　者：习岗[1] 贺瑞瑞[1] 刘锴[1] 赵燕燕[1] 

机构地区：[1]西安理工大学理学院应用物理系,西安710048

出　　处：《农业工程学报》2015年第3期319-324,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(51277151;31471412);西安理工大学科技创新计划项目(2013CX019)

摘　　要：为了揭示极低频脉冲电场(extremely low frequency pulsed electric field,ELF-PEF)对作物幼苗抗旱性的影响及其机理,采用渗透势为-0.1MPa的PEG-6000溶液对玉米幼苗形成生理干旱,研究了1Hz脉冲电场对渗透胁迫下玉米幼苗生长和自发光子辐射的变化。结果表明,在-0.1 MPa的渗透胁迫下玉米幼苗根系和叶片鲜质量缓慢增长,玉米幼苗种胚的自发光子辐射表现出先增加后减小的趋势,幼苗根系和叶片的自发光子辐射也呈现出波动性的增长趋势;经过脉冲电场处理的玉米幼苗根系和叶片鲜质量始终高于(P<0.05)未经过脉冲电场处理的对照组,玉米种胚的自发光子辐射峰值出现的时间比对照组早(P<0.01),峰值辐射强度也比对照高(P<0.01),玉米幼苗根系和叶片的自发光子辐射都有所增强(P<0.05),在渗透胁迫初期脉冲电场处理对玉米幼苗叶片自发光子辐射的增强效应非常显著(P<0.01)。研究结果为揭示极低频脉冲电场对作物幼苗抗旱性的影响机理提供参考。There are many complex physiological and biochemical reactions in the process of crop seed germination, so germinating seeds are very susceptible to environmental changes and have significant changes on the later growth. Using various physical methods to improve crops drought resistance has been one of the important research directions of modern agriculture, and the majority of studies have been focused on the high-voltage electrostatic field. Recent studies showed that the center frequency of corn cell potential fluctuations was in near 1 Hz, the cell potential coupling resonance could be occurred under the action of 1 Hz pulsed electric field, and some key physiological processes such as respiratory metabolism, photosynthesis, stomatal conductance and water uptake could be affected by the extremely low frequency pulsed electric field（ELF-PEF） with 1 Hz. Therefore, the effect of 1 Hz pulsed electric field on crop drought resistance could be greater than the electrostatic field. However, the reports about the effect of the ELF-PEF on crop drought resistance and its mechanism have been very little, and hence the corresponding researches need to be strengthened. Spontaneous photon emission is an important life information. Numerous studies have determined that the spontaneous photon emission from living cells is closely related to some important life processes such as cell metabolism, DNA synthesis, respiration and photosynthesis. The analysis of spontaneous photon emission from living cells could provide a non-invasive optical technology for the study of changes in the cell metabolism and the function of the state, and it has been widely used in the studies of plant reaction to pathogens, flooding, drought, salt stress, herbicides, UV radiation and so on. For the above reasons, after the germinating corn seeds were treated by 1 Hz ELF-PEF and the PEG-6000 solution was used to form physiological drought to corn seedlings, the growth of corn seedlings and spontaneous photon emission under osmotic stress were stu

关 键 词：电场 作物 辐射 渗透胁迫 幼苗抗旱性 玉米 

分 类 号：Q632[生物学—生物物理学]