纤维乙醇发酵残渣中酶解木质素的提取与表征  

Extraction and characterization of enzymatic hydrolysis lignin from cellulosic ethanol fermentation residue

作　　者：潘奇[1,2] 陈介南[1] 张新民[2] 詹鹏[1] 张林[1] 

机构地区：[1]中南林业科技大学国家林业局生物乙醇研究中心,湖南长沙410004 [2]中南林业科技大学材料科学与工程学院,湖南长沙410004

出　　处：《化工进展》2015年第1期86-90,159,共6页Chemical Industry and Engineering Progress

基　　金：国家林业公益性行业科研专项(201404604);湖南省科技计划重点(2014WK2027);国家林业科学技术推广项目([2014]55号)

摘　　要：近年来,将纤维素乙醇生产过程中所产生的废物进行资源化利用已越来越受重视。本研究利用杨木纤维酶解发酵产纤维素乙醇的残渣进行木质素的提取与表征,采用单因素试验分析碱浓度、料液比、反应温度、反应时间对酶解木质素提取效果的影响,并对反应条件进行正交优化,应用UV、FT-IR光谱仪对分离出的酶解木质素结构进行表征。结果表明:酶解木质素最佳的提取工艺条件为Na OH浓度40g/L、料液比1∶30、反应温度60℃、反应时间2.5h。紫外和红外光谱显示酶解木质素保留了完好的木质素结构,以紫丁香基木质素为主,有良好的化学活性。The resource of the wastes from cellulosic ethanol production gets more and more attention. Enzymatic hydrolysis lignin（EHL） was extracted and characterized from the residue of bioethanol fermentation using enzymatic poplar fiber. The single factor experiment analysis was conducted to study the effect of alkali concentration, solid-liquid ratio, reaction temperature and time on extraction of EHL. Reaction conditions were optimized by orthogonal analysis. The structure of EHL was characterized using ultraviolet（UV）,Fourier Transform-Infrared Spectroscopy（FT-IR）. The optimum EHL extraction conditions were Na OH 40g/L, solid-liquid ratio 1 ∶ 30, reaction temperature 60℃ and reaction time 2.5h. UV and FT-IR spectra showed that the EHL retained intact lignin structure,mainly with syringyl lignin,holding high chemical activities.

关 键 词：发酵残渣 酶解木质素 木质纤维 结构表征 纤维乙醇 

分 类 号：TQ31[化学工程—高聚物工业]