胆囊收缩素对急性胰腺炎大鼠肠Cajal间质细胞及小肠运动功能的影响  

Effect of cholecystokinin on intestinal interstitial cells of Cajal and small intestinal motor function in acute pancreatitis rats

作　　者：高孝朋 吴兴茂[1] 臧彬[1] 

机构地区：[1]中国医科大学附属盛京医院ICU,沈阳110004

出　　处：《中华临床营养杂志》2015年第3期151-155,共5页Chinese Journal of Clinical Nutrition

摘　　要：目的探讨胆囊收缩素（CCK）对急性胰腺炎（AP）大鼠肠Cajal间质细胞（ICC）网络结构及小肠运动功能的影响。方法将sD雄性大鼠20只按随机数字表法分为模型组和对照组：模型组15只，用于建立急性胰腺炎模型，并按CCK剂量分为AP组、AP＋CCK20组（CCK20μg/kg）、AP＋CCK40组（CCK40μg/kg）3个亚组；正常对照组5只。模型组大鼠采用L-鸟氨酸腹腔注射法造模，正常对照组同时间点给予生理盐水腹腔注射。造模24h后按各亚组剂量给予CCK腹腔注射，连续5d。造模6d后，每只大鼠在清醒状态下将墨水2ml灌入胃内，30min后颈椎脱臼法处死大鼠，迅速剖腹，测量变色部分小肠长度及小肠全长，计算二者比值；并取回肠组织行免疫荧光染色及激光共聚集扫描，比较各组阳性细胞数。结果对照组肌间丛ICC数量为（21．16±3．19）个／视野，深肌丛ICC数量为（17．20±1．75）个／视野；AP组肌间丛ICC数量为（5．00±1．05）个／视野，深肌丛ICC数量为（4．52±1．05）个／视野；AP＋CCK20组肌间丛ICC数量为（10．76±2．09）个／视野，深肌丛ICC数量为（9．84±1．68）个／视野；AP＋CCK40组肌间丛ICC数量为（13．72±2．97）个／视野，深肌丛ICC数量为（12．40±1．81）个／视野；AP组ICC数量较对照组显著下降（P=0．001）。正常对照组传输距离为0．71±0．05，AP组为0．54±0．07，AP＋CCK20组为0．68±0．10，AP＋CCK40组为0．74±0．07，AP组小肠运动功能减弱，传输距离变短，使用CCK后有所改善（P=0．003）。结论胰腺炎能够引起ICC损伤，ICC数量明显减少，导致ICC网络结构破坏，小肠运动功能下降，使用CCK后能够减轻ICC损伤，保护ICC网络结构的完整性，从而改善小肠运动。Objective To investigate the effect of cholecystokinin （CCK） on rat intestinal interstitial cells of Cajal （ ICC ） and the small intestinal motor function during experimentally induced acute pancreatitis （AP）. Methods 20 male SD rats were divided with a random number table into model group （ n = 15 ） and control group （ n = 5 ） ： the 15 rats in the model group were further divided into 3 subgroups, i.e. 0, 20, 40 μg/kg CCK sub-groups. L-omithine was intraperitoneally injected to induce pancreatitis, while normal saline was injected in the control group. 24 hours after establishing AP model, CCK was administered intraperitoneally every day for 5 consecutive days. The rats were sacrificed by cervical dislocation 30 minutes after gavage with 2 ml ink. Length of the small intestine which have become black and that of the whole small intestine were measured. Intestinal sections were studied by confocal microscopy after immunofluorescence with specific antibodies. The number of positive cells was compared among the groups. Results In the control group, the number of ICC in the myenteric plexus and deep muscular plexus was （ 21.16 ± 3. 19 ） /field and （ 17. 20 ±1.75） /field, respectively; in the AP group was （5.00 ± 1.05） /field and （4. 52 ± 1.05） /field, respectively; in the AP ＋ CCK20 group was （ 10. 76 ± 2.09） /field and （9.84 ± 1.68） /field, respectively; in the AP ＋ CCK40 group was （ 13.72 ±2. 97） /field and （ 12.40 ±1.81 ） /field, respectively; the number of ICC in the AP group were significantly lower than those in the control group （P = 0. 001 ）. The ratio of transmission distance in small intestine in the control, AP, AP ＋ CCK20, and AP ＋ CCK40 groups was 0. 71 ±0.05, 0. 54 ± 0. 07, 0. 68 ± 0. 10, and 0. 74 ± 0. 07, respectively. The intestinal motor function was reduced in the AP group, demonstrated by shortened transmission distanee, which was improved after the administration of CCK （P =0. 003）. Conclusions Panereatitis can ca

关 键 词：胆囊收缩素 CAJAL间质细胞 胰腺炎 小肠运动 

分 类 号：R285.5[医药卫生—中药学]