钙蛋白酶-10基因SNP-19与多囊卵巢综合征相关性研究  

Association of CAPN-10 SNP-19 with polycystic ovary syndrome

作　　者：邓妙[1,2] 刘元伟 张红艳[1] 岑加萍[1] 张治芬 

机构地区：[1]杭州市第一人民医院妇产科,310006 [2]杭州市妇产科医院

出　　处：《浙江医学》2015年第11期912-915,930,共5页Zhejiang Medical Journal

基　　金：浙江省科技计划项目(2009C33114)

摘　　要：目的 探讨钙蛋白酶-10基因（CAPN-10）单核苷酸多态性-19（SNP-19）与多囊卵巢综合征（PCOS）的相关性.方法 采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术,对107例PCOS患者（PCOS组）及11 1例健康体检者（对照组）的CAPN-10 SNP-19进行分析,并测定其性激素和生化指标.PCOS组再根据BMI与胰岛素抵抗（IR）稳态模型指数（HOMA-IR）分为肥胖组与非肥胖组、IR组与非IR组,分析CAPN-10 SNP-19与其关系.结果 与对照组相比,PCOS组BMI、空腹血糖（FPG）、TG、TC、LDL、黄体生成素（LH）、黄体生成素/卵泡刺激素（LH/FSH）、睾酮（T）均升高（均P＜0.01）.PCOS组CAPN-10 SNP-19 1等位基因及1/2基因型频率均高于对照组（均P＜0.05）;且CAPN-10 SNP-19 1/2基因型PCOS发病风险较1/1、2/2基因型增加约2倍（OR=1.9,95％CI=1.1～3.3,P＜0.05）.CAPN-10 SNP-19 1、2等位基因频率PCOS肥胖组与非肥胖组、IR组与非IR组比较均无统计学差异（均P ＞0.05）;PCOS肥胖组和IR组CAPN-10 SNP-19 1/2基因型频率分别高于非肥胖组和非IR组,但差异均无统计学意义（均P＞0.05）.结论 CAPN-10 SNP-19与PCOS遗传易感性有关,可能与PCOS肥胖及IR无关.Objective To investigate the relationship between single nucleotide polymorphisms-19 （SNP-19） in calpain-10 （CAPN-10） gene and polycystic ovary syndrome （PCOS）. Methods One hundred and seven PCOS patients（PCOS group） and 111 healthy female subjects（control group） were recruited in the study. The distribution of CAPN-10 SNP-19 was an- alyzed by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP）. Serum hormones and biochemical indicators were also measured. Oral glucose tolerance test （OGTT） was performed in PCOS group. Results The BMI, FPG, TG, TC, LDL, LH, LH/FSH and T levels were significantly increased in PCOS group compared to control group（P〈0.01）.The frequency of allele 1 and genotype 1/2 of CAPN-10 SNP-19 were higher in PCOS group than those in control group. Genotype 1/2 was closely associated with PCOS compared to 1/1 and 2/2（0R=1.9, 95%C1=1.1-3.3, P〈0.05）. There was no significant difference in frequency of genotype 1/2 between obese group and non-obese group, between insulin resistance （IR） group and non-IR group of PCOS patients （P 〉0.05）. Conclusion The SNP-19 in CAPN-10 may contribute to the genetic susceptibility to PCOS, but it may be not correlate with obesity or IR of PCOS patients.

关 键 词：多囊卵巢综合征 半胱氨酸内肽酶类 钙蛋白酶-10 多态性 单核苷酸 胰岛素抵抗 

分 类 号：R711.75[医药卫生—妇产科学]