猪初乳和常乳的差异蛋白质组成分析  

Differential Protein Composition Analysis of Sow Colostrum and Mature Milk

作　　者：吴伟宗[1] 姜明涛 刘闯[1] 王晓秋[1] 赖长华[1] 王凤来[1] 王军军[1] 

机构地区：[1]中国农业大学动物科技学院,动物营养学国家重点实验室,北京100193 [2]辽宁省盘锦市于楼92538部队农副业基地,辽宁盘锦124120

出　　处：《中国畜牧杂志》2015年第13期25-29,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金(u0731001;31272449;31172216)

摘　　要：本文旨在分析猪初乳和常乳乳清中的蛋白质组成,以揭示猪不同泌乳阶段的乳蛋白质组成特性。选用6头健康初产达兰母猪,分别在分娩后第1天和第14天,颈部注射催产素,采集前后乳区混合的初乳和常乳。将全乳样经过差速离心后获得乳清,利用2-DE结合MALDI-TOF-MS的蛋白质组学技术对初乳和常乳的乳清进行比较分析。结果表明:初乳中相对含量较高的蛋白点15个(P<0.05),主要为Ig G、s Ig A和s Ig M的片段、α-乳清白蛋白和触珠蛋白;常乳中相对含量较高的蛋白点共有16个(P<0.05),主要为s Ig A和s Ig M的片段、叶酸结合蛋白、补体因子-D以及一些来自乳腺上皮细胞的蛋白。由此可见,初乳和常乳的差异主要表现在免疫球蛋白以及免疫调节因子上,并发现具有免疫调节作用的触珠蛋白在初乳中相对含量较高。从以上结果可以看出,初乳中高丰度的Ig G、s Ig A、s Ig M以及触珠蛋白为新生仔猪的被动免疫提供有力的保障,随着新生仔猪生长发育,常乳中的s Ig A和s Ig M依然在新生仔猪粘膜免疫方面发挥重要作用。The objective of this study was to investigate the differentially expressed proteins between sow colotrum and milk. Six healthy primiparous sows were used in the experiment. At d 1 and 14 postpartum, colostrum and milk were collected respectively after the sows were treated with oxytocin. Whey was prepared by removing the casein and fat from the whole milk, and frozen at -70℃ until analyzed. Comparative proteomics with two-dimensional gel electrophoresis （2-DE） and matrix assisted laser desorption/ionization-time of flight-mass spectrometry （MALDI- TOF-MS）, was performed to identify the differentially expressed proteins between colostrum and mature milk. Results show that relative concentrations of 15 protein spots were higher in colostrum compared with milk, including immunoglobulins, haptoglobins and ct-lacta｜bumin; sixteen proteins, such as immunoglobulins, folate binding protein, complement factor D and some proteins from somatic cells, were increased expression in mature milk compared with colostrum. Immunoglobulins, especially IgG and haptoglobin, in colostrum provide passive immunity for neonatal piglets, while sIgA and sIgM became the main immunoglobulin in mature milk, and continued protected newborn from infectious agents.

关 键 词：母猪 初乳 常乳 蛋白质组学 

分 类 号：S828.5[农业科学—畜牧学]