基于SSR技术对七十五份栽培葡萄品种的鉴定研究  

Identification of SSR in 75 Cultivated Grape Varieties

作　　者：李雪雁[1] 梁海永[1] 宪立杰[1] 杨敏生[1] 

机构地区：[1]河北农业大学,河北省林木种质资源与森林保护重点实验室,河北保定071000

出　　处：《北方园艺》2015年第13期115-119,共5页Northern Horticulture

基　　金：国家林业局林业公益性行业科研专项资助项目(201104039)

摘　　要：以75份葡萄栽培品种为试材，采用SSR分子标记技术，研究建立一套适合于葡萄DNA指纹图库构建的技术体系，为葡萄种质资源的鉴定和亲缘关系分析提供依据。结果表明：选用19对核心引物，共扩增出89个多态性位点，利用DPS软件，对其进行聚类分析，75份葡萄种质的遗传相似系数在0．0283～0．8873，平均遗传相似系数为0．47539。依据用途，初步分为鲜食葡萄和酿酒葡萄两大类，并根据品种来源，大致分为了欧美杂种、美洲杂种和欧亚种。In this experiment,75 parts of grape cultivars were selected as test materials,using SSR molecular markers to study the establishment of a suitable grape DNA fingerprinting technology to build library system for the identification and genetic relationship grape germplasm resources,to provide the basis for analysis. The results showed that the choice of 19 pairs of core primers were amplified 89 polymorphic loci by DPS software,its cluster analysis,75 parts of the grape germplasm genetic similarity coefficient 0. 028 3-0. 887 3 average genetic similarity coefficient was 0. 475 39. According to use,initially divided into table grape and wine grape into two categories, depending on the variety and origin, divided into the European and American hybrids, American hybrids and Eurasian species.

关 键 词：葡萄 SSR标记 聚类分析 

分 类 号：S663.102.4[农业科学—果树学]