益气温阳活血方含药血清对肥大心肌细胞β_3-肾上腺素能受体的影响研究  

Effects of serum containing Yiqiwenyang-huoxue Decoction on β_3-adrenergic receptor in hypertrophic cardiomyocytes

作　　者：王里[1] 顾广富 罗建华[1] 杨冬花 

机构地区：[1]贵州省人民医院干部医疗科,贵州贵阳550002 [2]贵阳中医学院,贵州贵阳550025

出　　处：《中国现代医学杂志》2015年第17期22-25,共4页China Journal of Modern Medicine

基　　金：贵州省社会公关项目[No:黔科合SY(2011)3055];贵州省科技联合基金[No:黔科合SY(2010)3121]

摘　　要：目的探讨益气温阳活血方含药血清对β3-肾上腺素能受体(β3-AR)的影响。方法通过血管紧张素Ⅱ(AngⅡ)诱导肥大心肌细胞,以3H-亮氨酸掺入量检测心肌细胞蛋白质合成速率,酶联免疫吸附(ELISA)法检测细胞上清去甲肾上腺素(NE)、金属基质蛋白酶-2(MMP-2)、金属基质蛋白酶-9(MMP-9)、金属基质蛋白酶抑制物-1(TIMP-1)、金属基质蛋白酶抑制物-2(TIMP-2)含量,以逆转录聚合酶链反应(RT-PCR)法以及蛋白质印记法(Western blot)检测β3-AR m RNA及蛋白质表达水平。结果与正常组比较,模型组心肌细胞蛋白质合成速率、NE、MMP-2、MMP-9含量、β3-AR m RNA和蛋白质的表达水平明显升高(P<0.05),而TIMP-1、TIMP-2含量则明显降低(P<0.05);益气温阳活血方能够明显降低细胞蛋白质合成速率、NE、MMP-2、MMP-9含量,升高TIMP-1、TIMP-2含量,抑制β3-AR m RNA和蛋白质的表达,与正常组比较,差异无统计学意义(P>0.05)。结论益气温阳活血方可抑制心肌细胞肥大,其机制与干预β3-AR表达有关。【Objective】 To investigate the effects of serum containing Yiqiwenyang-huoxue Decoction onβ3-adrenergic receptor（AR） in the hypertrophic cardiomyocytes. 【Methods】 The hypertrophic cardiomyocyte model was induced by stimulation with Ang Ⅱ in vitro. The protein synthesis was assayed with 3H-leucine incorporation method. The content of NE, MMP-2, MMP-9, TIMP-1 and TIMP-2 was detected by ELISA.The expression levels of β3-AR m RNA and protein were determined by RT-PCR and Western blot analysis,respectively. 【Results】 Compared to the normal group, the protein synthesis and the content of NE, MMP-2and MMP-9 were significantly enhanced; the β3-AR m RNA and protein expression levels were also up-regulated, but TIMP-1 and TIMP-2 content was significantly lower in the hypertrophic cardiomyocyte group（P〈 0.05）. Those were attenuated by Yiqiwenyang-huoxue Decoction without significant differences（P〉 0.05）.【Conclusions】Yiqiwenyang-huoxue Decoction may inhibit cardiomyocyte hypertrophy through β3-AR.

关 键 词：益气温阳活血方 去甲肾上腺素 心肌肥大 Β3-肾上腺素能受体 

分 类 号：R286[医药卫生—中药学]