乙二胺功能化修饰多壁碳纳米管及对PC12细胞生物相容性的研究  

Preparation of Ethylenediamine Functionally-Modified Multi-Walled Carbon Nanotubes and Biocompatibility on PC12 Cells

作　　者：张涛[1] 韩博[1] 张华[1] 陈文[1] 

机构地区：[1]石河子大学药学院,新疆石河子832000

出　　处：《中国药学杂志》2015年第13期1123-1129,共7页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81160395)

摘　　要：目的乙二胺(EDA)功能化修饰多壁碳纳米管(MWCNTs-COOH)制备低毒、分散性良好的多壁碳纳米管-乙二胺复合物(MWCNTs-EDA),为碳纳米管作为药物载体研究奠定一定基础。方法乙二胺功能化修饰多壁碳纳米管和乙二胺通过共价交联法制备得到多壁碳纳米管-乙二胺复合物,利用扫描电镜(SEM)、透射电镜(TEM)、红外光谱(FTIR)、热失重分析(TG)、拉曼光谱(Raman)、Zeta电位对复合物进行表征及对修饰前后的碳纳米管分散性进行考察;采用MTT法及流式细胞仪法考察其对大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)的毒性作用,评价复合物体外生物相容性。结果扫描电镜、透射电镜、红外光谱、热失重分析、拉曼光谱、Zeta测定结果证实乙二胺成功接枝到多壁碳纳米管上;分散性实验结果表明,多壁碳纳米管-乙二胺复合物在去离子水中分散均匀且稳定性良好;MTT及流式细胞实验结果表明,通过乙二胺功能化修饰多壁碳纳米管使得多壁碳纳米管的细胞毒性显著降低。结论乙二胺功能化修饰后的多壁碳纳米管分散性得到明显改善且对细胞毒性显著降低,为碳纳米管作为新载体的进一步研究提供有益依据。OBJECTIVE To prepare multi-walled carbon nanotubes-ethylenediamine（ MWCNTs-EDA） compound with low toxicity and better dispersion using EDA functionally modified-multi-walled carbon nanotubes to lay a foundation for the further research of loading drugs with carbon nanotubes.METHODS MWCNTs-EDA compound was synthesized by covalent crosslinking with carboxylated carbon nanotubes（ MWCNTS-COOH） and ethylenediamine,and then scanning electron microscopy（ SEM）,transmission electron microscopy（ TEM）,infrared spectroscopy（ FTIR）,thermal gravimetric analysis（ TG）,Raman spectrum,and Zeta potential determination were used to characterize the compound,and the dispersion uniformity of MWCNTs before and after functionalization were examined.MTT assay and flow cytometry test were used to measure the toxicity of the compound against phcochromocytoma cells.RESULTS The results of SEM,TEM,FTIR,TG,Raman,and Zeta potential analysis confirmed that MWCNTs had successfully received the EDA.The dispersibility test showed that MWCNTs-EDA had better dispersion stability in water.MTT assay and flow cytometry test showed that EDA functionally-modified MWCNTs greatly reduced the cytotoxicity of the MWCNTs.CONCLUSION The EDA functionally-modified MWCNTs has significantly improved dispersibility,significantly reduced in vitro cytotoxicity,and better biocompatibility.

关 键 词：多壁碳纳米管 乙二胺 细胞毒性 

分 类 号：R944[医药卫生—药剂学]