异丙酚对大鼠脊髓γ-氨基丁酸A、N-甲基-D天门冬氨酸受体表达的影响  

Effect of propofol on gamma aminobutyric acid A and N methyl D aspartate receptor expression in spinal cord of rats

作　　者：王志涛[1] 刘德君[1] 董铁立[1] 

机构地区：[1]郑州大学第二附属医院麻醉科,450014

出　　处：《中华实验外科杂志》2015年第7期1510-1512,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察异丙酚对大鼠脊髓γ-氨基丁酸A（GABAA）、N-甲基-D天门冬氨酸受体（NMDA）表达的影响.方法 健康雄性SD大鼠40只,体质量220～ 250 g.随机分为空白对照组（C）、生理盐水组（NS）、异丙酚组（P组）,P组分为P1、P2、P3组.生理盐水组（NS组）,腹腔注射生理盐水;异丙酚组,腹腔注射异丙酚5 mg/kg（P1组）、30 mg/kg（P2组）、100 mg/kg（P3组）.5 min后大鼠右侧后肢趾部皮下注射5％的福马林100μl建立疼痛模型,记录大鼠缩腿、舔爪累计时间.2h后处死大鼠,取脊髓使用免疫组织化学方法测定GABAA、NMDA的积分吸光度（IA）值.结果 C组没有缩腿、舔爪反应,与NS组[（1 326.56±198.32）s]比较,P组累计时间明显缩短（P＜0.05）;P3组（170.34±1.21） GABAA的IA值明显的高于其余4组,C组（85.86 ±0.47）最低.与C组比较,N、P组显著增高（P＜0.05）;C组（92.17 ±0.81） NMDA的IA值最低,NS组（190.43±1.20）最高,P组中P3最低.组间比较差异有统计学意义（P＜0.05）.结论 脊髓GABAA、NMDA受体参与了异丙酚的镇痛作用.Objective To observe the effects of propofol on the expression of N methyl D aspartate receptor （NMDA） and gamma aminobutyric acid A （GABAA） in the spinal cord of rats.Methods Healthy SD rats were randomly divided into 5 groups：control group （n =8） without given any treatment,normal saline group （n =8）,and propofol group [n =24,subdividing 3 propofol subgroups （n =8 each）]：concentrations of propofol 5,30 and 100 mg/kg respectively）.Five min later,5％ formalin （100 μl） was injected subcutaneously into the planta region of right hindpaw.Rats were killed after 2 h and lumbar spinal cord was removed.The expression of GABAA and NMDA receptor proteins in the spinal cord dorsal horn was detected.Results The expression of GABAA protein in propofol 100 mg/kg subgroup （170.34 ± 1.21） was significantly higher than that of the rest 4 groups with the highest expression in control group （85.86 ± 0.47）.As compared with control group,the GABAA protein expression was significantly increased in normal saline group and propofol group （P ＜ 0.05）.Integral absorbance of NMDA in control group was lowest （92.17 ± 0.81）,and that normal saline group was highest （190.43 ± 1.20）.The expression of NMDA protein was highest in propofol 100 mg/kg subgroup,and there was significant difference among three propofol subgroups （P ＜ 0.05）.Conclusion Spinal GABAAR and NMDAR receptors may participate in the analgesic effect of propofol.

关 键 词：异丙酚 γ-氨基丁酸A N-甲基-D天门冬氨酸受体 

分 类 号：R285.5[医药卫生—中药学]