新型纳米材料乳酸-羟基乙酸-L-赖氨酸多肽接枝聚/聚乳酸/纳米羟基磷灰石/丙戊酸神经导管促进大鼠周围神经再生的研究  

Effect of new nanocomposite RGD peptide conjugated poly [LA-（Glc-Lys）]/poly （D, L-lactic acid）/nanosized hydroxyapatite/valproic acid nerve conduits on peripheral nerve regeneration in a rat model

作　　者：吴飞[1] 李亚明[1] 张卫兵[1] 胡戈亮[1] 刘世清[1] 勘武生[2] 

机构地区：[1]武汉大学人民医院骨外科,430060 [2]武汉市普爱医院骨外科

出　　处：《中华实验外科杂志》2015年第7期1595-1598,共4页Chinese Journal of Experimental Surgery

基　　金：湖北省卫生厅青年科技人才项目（QJX2012-37）

摘　　要：目的 观察新型纳米复合材料乳酸-羟基乙酸-L-赖氨酸多肽接枝聚/聚乳酸/纳米羟基磷灰石/丙戊酸（PRGD/PDLLA/n-HAP/VPA）神经导管对周围神经缺损的影响.方法 30只大鼠随机分成3组,A组用PRGD/PDLLA/n-HAP/VPA导管桥接缺损神经,B组用PDLLA导管进行桥接,C组行自体神经移植术.结果 术后12周,A组（-41.00±3.08）和C组（-39.00 ±3.12）坐骨神经功能指数（SFI）高于B组（-73.00 ±3.11,P＜0.05）;A组（24.78 ±4.05）和C组神经潜伏期波幅（CMAPs,25.39±5.54） mV高于B组[（14.74 ± 3.09） mV,P＜0.05];A组（31.39 ±2.38） m/s和C组（31.53±2.11）m/s传导速度（NCVs）高于B组[（20.07 ±2.31） m/s,P＜ 0.05];A组（256.00 ±6.08）和C组（258.00 ±5.49）的导管段神经纤维计数显著高于B组（231.00±5.16,P＜0.05）,A组与C组之间差异无统计学意义（P＞0.05）.结论 新型PRGD/PDLLA/n-HAP/VPA复合材料神经导管有望应用于周围神经缺损疾病的临床治疗.Objective To observe the enhancing effect of nerve regeneration on peripheral nerve defect models bridged by a new RGD peptide conjugated poly [LA-（Glc-Lys）]/Poly （D,L-lactic acid）/nanosized hydroxyapatite/valproic acid （PRGD/PDLLA/n-HAP/VPA） nanocomposite conduit.Methods In this study,30 rats were randomly divided into 3 groups （n =10）：group A,PRGD/PDLLA/n-HAP/VPA;group B,PDLLA;group C：autograft.Results At 12th week after surgery,the sciatic functional index （SFI） values in groups A （-41.00±3.08） and C （-39.00 ±3.12） were significantly higher than those in group B （-73.00 ± 3.11,P ＜ 0.05）.The compound muscle action potentials （CMAPs,mV） in groups A （24.78 ± 4.05） and C （25.39 ± 5.54） were significantly higher than those in group B （14.74 ±3.09,P＜0.05）.The nerve conduction velocity studies （NCVs,m/s） in groups A （31.39 ±2.38） and C （31.53 ±2.11） were significantly higher than those in group B （20.07 ± 2.31,P ＜0.05）.The number of regenerated nerve in the tube of groups A （256.00 ±6.08） and C （258.00 ± 5.49） was significantly greater than that of group B （231.00 ±5.16,P ＜0.05）.There was no significant difference between groups A and C （P ＞ 0.05）.Conclusion These promising results illustrate that this PRGD/PDLLA/n-HAP/VPA nanocomposite conduit has great potential for clinical applications.

关 键 词：组织工程 周围神经再生 神经导管 丙戊酸 

分 类 号：R318.08[医药卫生—生物医学工程]