灯盏花及其药物灯盏花素中酚类成分的鉴定和含量测定(英文)  

Identification and Quantification of Phenolic Components of Erigeron breviscapus and Its Derived Drug Breviscapine

作　　者：张人伟 樊献俄 李来伟 林隆泽[2] James M.Harnly 

机构地区：[1]昆明龙津药业股份有限公司,昆明650091 [2]美国农业部农业研究服务Beltsvi]]e人类营养研究中心食品成分和测定方法研究实验室,USA20705

出　　处：《天然产物研究与开发》2015年第6期962-970,共9页Natural Product Research and Development

基　　金：supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health

摘　　要：本研究采用一个标准高效液相色谱质谱(HPLC-PDA-ESI/MS)分析方法和酚类成分定量方法确定了灯盏花中的33个肉桂酸衍生物和16个黄酮类化合物,灯盏花素中18个黄酮类化合物。使用干燥后的绿原酸(326 nm)作为标准品,根据326 nm处紫外吸收峰相对强度和克分子(mole)相对响应因子(MRRF)确定了每个肉桂酸衍生物的含量。以同样的方式,使用干燥后的芹菜素(336 nm)和芦丁(354 nm)确定了8个芹菜素,3个木犀草素,3个黄酮醇3-糖苷和7-葡萄糖醛酸苷的含量。这是首次报道十余微量黄酮类化合物存在于灯盏花素,以及灯盏花全植物中每个酚类化合物的含量。A standardized HPLC-PDA-ESI / MS profiling method was used to identify the phenolic components of Erigeron breviscapus and breviscapine. A general phenolic quantification method was used to quantify the components. As a result,33 hydroxycinnamates and 16 flavonoids were detected in E. breviscapus,a plant used in traditional Chinese medicine for treating cardiocerebral diseases. Eighteen flavonoids were detected in breviscapine,an E. breviscapus derived drug. The hydroxycinnamates were quantified using HPLC-PDA at the detection wavelength of 326 nm and the mole relative response factors（ MRRF）. Dried chlorogenic acid was used as calibration standard. Similarly,8 apigenins,4 luteolins,3 flavonol 3-O-glycosides,and the 7-O-glucuronides of quercetin and isorhamnetin were quantified using apigenin（ at 336 nm） and rutin（ at 354 nm） as calibration standards and MRRF value for each compound. This was the first detection of over 10 flavonoids in breviscapine,and the quantification of each phenolic component in E. breviscapus.

关 键 词：灯盏花全草 灯盏花素 肉桂酸衍生物 黄酮苷 HPLC-PDA-ESI/MS 含量测定 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]