CD69分子在人外周血佛波醇酯＋离子霉素活化的γδT细胞表面表达的实验研究  

CD69 expression on γδT cells activated by phorbol myristate acetate and ionomycin in human peripheral blood

作　　者：晋红梅[1] 张婷婷[2] 王克强[2] 朱莉[1] 吴家锋[2] 段衍超[3] 

机构地区：[1]泰山医学院附属医院输血科,泰安271000 [2]泰山医学院附属医院检验科,泰安271000 [3]泰山医学院附属医院血液科,泰安271000

出　　处：《国际免疫学杂志》2015年第4期321-324,共4页International Journal of Immunology

基　　金：山东省泰安市科技发展计划（201440774-38B）;山东省高等学校科技计划（J14LL03）

摘　　要：目的观察CD69分子在人外周血佛波醇酯（PMA）＋离子霉素（IM）活化的γδT细胞表面的表达情况。方法分离获取健康人外周血单个核细胞（PBMC），用PMA＋IM刺激，采用流式细胞术检测CD3^＋细胞和γδT细胞0、6、12、24、48和72h CD69分子的动态表达情况。结果PMA＋IM刺激PBMC0、6、12、24、48和72h，CD3^＋细胞CD69分子的表达分别为1．0％、99．5％、99．1％、98．0％、93．3％、92．1％；γδT细胞CD69分子的表达分别为0．91％、99．3％、98．6％、93．6％、88．7％、87．0％。结论PMA＋IM的刺激后CD3^＋细胞和γδT细胞迅速活化，CD69的表达几乎为100％，6h达高峰，随后缓慢下降，两种细胞活化表达CD69基本一致。Objective To observe the expression of CD69 molecules in γδT cells activated by human peripheral blood phorbol myristate acetate （PMA） and ionomycin （IM）. Methods Healthy human PBMC was isolated. PMA and IM were used to stimulate PBMC. We used flow cytometry to detect the dynamic expression of CD69 in CD3^＋ T cells and γδT cells after0,6,12,24,48 and 72 hours. Results PBMC was stimulated by PMA and IM for 0,6,12,24,48 and 72 hours. After that, CD69 expression in CD3^＋ T cells was 1.0% ,99. 5%, 99. 1% ,98.0% ,93. 3% and 92. 1%, respectively. CD69 expression in γδT cells was 0.91% ,99. 3%, 98. 6% ,93.6% ,88.7% and 87.0% ,respectively. Conclusion After being stimulated by PMA and IM ,CD3^＋ T cells and γδT cells were activated rapidly. The expression of CD69 was close to 100%, and the highest expression was found at 6 hours. After 6 hours, the CD69 expression fell down slowly. The similarly improve effect of CD69 expression was observed in both CD3^＋ T cells and γδT cells.

关 键 词：佛波醇酯 离子霉素 ΓΔT细胞 CD69分子 

分 类 号：R392[医药卫生—免疫学]