桃金娘组织培养初步研究  

Primary Study on Tissue Culture of Rhodomytrus tomentosa

作　　者：王尚显 杨光穗[1] 曾淇[3] 陈金花[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所海南省热带观赏植物种质创新利用工程技术研究中心农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]海南大学科技应用技术学院,海南儋州571737 [3]海南省农业学校,海南海口571100

出　　处：《湖南农业科学》2015年第4期74-77,共4页Hunan Agricultural Sciences

基　　金：海南省重大科技项目(ZDZX2013012);海南省星火产业带项目(HNXH201427);海南省重点科技计划项目(ZDXM20120025)

摘　　要：以桃金娘茎段、叶片、种子为外植体,研究0.1%Hg Cl2和2%Na Cl O单独消毒和组合消毒的消毒效果,探索出适合不同外植体消毒的有效方法和时间;并对最佳的诱导培养基和无菌系建立途径进行筛选。实验结果表明,组合消毒和消毒效率明显优于单一消毒剂消毒,其中单一消毒剂消毒时0.1%Hg Cl2的消毒效果又比2%Na Cl O好;桃金娘茎段、嫩叶、老叶、果实的最佳消毒方式分别为:2%Na Cl O浸泡10 min+0.1%Hg Cl2浸泡8 min、2%Na Cl O浸泡10 min+0.1%Hg Cl2浸泡6 min、0.1%Hg Cl2浸泡10 min+2%Na Cl O浸泡8 min、0.1%Hg Cl2浸泡16 min。嫩叶较老叶和茎段更易诱导出愈伤组织,最佳的诱导培养基为2号培养基,愈伤组织诱导率达77.7%。无菌播种是桃金娘无菌系建立的最佳方式,萌发率高达75.0%。Using stem, leaf, seed of Rhodomytrus tomentosa as explants, the paper analyzed the disinfection effect of 0.1% HgCI： and 2% NaC10 alone and in combination, explored the effective explants disinfection method and time for Rhodomytrus tomentosa explants, and screened the optimal approach for induced medium. The research results showed that： the combination disinfection effect was better than alone disinfection, and that of 0.1% HgC12 better than 2% NaC10; the best disinfection method for stem, tender leaf, old leaf, fruit of Rhodomytrus tomentosa was 2% NaC10 immersed 10 rain ＋0.1% HgC12 immersed 8 min, 2% NaC10 immersed 10 rain＋0.1% HgC12 immersed 6 min, 2% NaCIO immersed 10 min＋0.1% HgC12 immersed 8 rain, 0.1% HgC12 immersed 16 min respectively. Tender leaf is more easily induced callus than old leaf and stem. The best induction medium is no. 2 media. Young leaves of callus induction rate was 77.7%. Sowing under aseptic condition is the best way to clone Rhodomytrus tomentosa, and the germination rate is as high as 75.0%.

关 键 词：桃金娘 组织培养 消毒 

分 类 号：S723.132[农业科学—林木遗传育种]