杀螺胺乙醇胺盐原药的反相高效液相色谱测定  

Determination of Niclosamide Ethanolamine Salt by RP-HPLC

作　　者：曹佳[1,2] 宋思思[1,2] 李晓刚[1,2] 刘一平 杨雪清 

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南省生物农药与制剂加工工程技术研究中心,湖南长沙410128 [3]湖南省农药检定所,湖南长沙410005 [4]湖南京西祥隆化工有限公司,湖南株洲412005

出　　处：《湖南农业科学》2015年第4期102-104,108,共4页Hunan Agricultural Sciences

基　　金：湖南省自然科学基金资助项目(13JJ5023);湖南省高等学校科学研究重点项目(11A053)

摘　　要：利用反相高效液相色谱法研究了两种色谱柱,3种流动相对杀螺胺乙醇胺盐分离效果的影响,并对测定方法进行比较。结果表明,以SpursilTMC18为色谱柱,流动相为甲醇︰水︰三乙胺=90︰10︰0.1(V/V)时,测定值的标准偏差为0.185,变异系数为0.240%,平均回收率为97.12%,线性相关系数为0.999 9。这说明在该条件下杀螺胺乙醇胺盐的分离效果较好,同时该方法简单快捷,可作为分析杀螺胺乙醇胺盐原药的分析方法。In order to analyze the influence of two kinds of chromatographic column, three types of mobile phase on the separating effect of Niclosamide ethanolamine salt, RP-HPLC was used. The results showed that when the chromatographic column was SpursilTM C 18, and the mobile phase of methanol, water and trimethylamine was 90 ： 10 ： 0.1 （V/V） ,the standard deviation of measured value was 0.185, the variable coefficient was 0.240%, the average recovery rate was 97.12% and the linearly dependent coefficient was 0.999 9. Therefore, we can conclude that under this condition the Niclosamide ethanolamine salt had good separating effect, and the method can be used to measure the active compound of Niclosamide ethanolamine salt because of its simple and rapid.

关 键 词：杀螺胺乙醇胺盐 氯硝柳胺 高效液相色谱法 三乙胺 

分 类 号：TQ450.7[化学工程—农药化工]