紫花鄂北贝母愈伤组织诱导及总生物碱的测定  

Callus induction and determination of total alkaloid content from Fritillaria ebeiensis var.purpurea

作　　者：刘群[1] 肖波[1] 李天祥[1] 李庆和[1] 张伯礼[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《中草药》2015年第12期1836-1839,共4页Chinese Traditional and Herbal Drugs

基　　金：天津市科技支撑计划项目(10ZCZDSY12400):生多调查(环):第四次全国中药资源普查天津试点

摘　　要：目的研究紫花鄂北贝母Fritillaria ebeiensis var.purpurea外植体愈伤组织诱导的激素组合和配比,并考察原药材及愈伤组织中总生物碱的量。方法以紫花鄂北贝母新鲜鳞茎为外植体,用抗生素和NaCIO组合作消毒剂,基于MS固体培养基添加激素2,4-D+6-BA、2,4-D+KT设计正交试验进行离体培养;采用可见-紫外分光光度法测定总生物碱量。结果外植体适宜灭菌条件是以5%NaClO溶液消毒8 min:愈伤组织诱导激素水平为2,4-D 0.5 mg/L+6-BA2.0mg/L;原药材及其愈伤组织中总生物碱量分别为1.46%、1.09%。结论愈伤组织质地疏松,适合继代培养,其有效成分总生物碱量低于原药材。Objective To study the combination and proportion for the explants in NaClO and the suitable hormone induced by callus of Fritillaria ebeiensis van purpurea.The total alkaloids content variations of raw herb and callus were investigated.Methods The fresh bulbs of F.ebeiensis var.purpurea were used as the exprimental materials and NaClO and antibiotics as disinfectants.It was cultured in vitro in MS medium with plant hormones 2,4-D ＋ 6-BA and 2,4-D ＋ KT by orthogonal test design.Alkaloids contents in original bulb and callus were measured with UV.Results The best way to sterilization was at a concentration of 5%NaClO with 8min.The optimal hormone levels was 2,4-D 0.5 mg/L ＋ 6-BA 2.0 mg/L.The alkaloid contents of raw herbs and callus were 1.46%and1.09%.Conclusion Callus induced by tissue culture technique are soft,luster,and transparent,which are suitable for subculture.Total alkaloids content in callus is less than that in raw herb.

关 键 词：紫花鄂北贝母 愈伤组织诱导 植物生长激素 总生物碱 激素 

分 类 号：R282.21[医药卫生—中药学]