辣椒疫霉多聚半乳糖醛酸酶活性对其致病力的影响  

Effect of Activities of Polygalacturonase on Pathogenicity in Phytophthora capsici

作　　者：李萍[1] 张茜茹[2] 吴亚[2] 高智谋[2] 

机构地区：[1]安庆职业技术学院园林园艺系,安徽安庆246003 [2]安徽农业大学植物保护学院,安徽合肥230036

出　　处：《安徽农业科学》2015年第19期111-113,167,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽省自然科学基金项目(070411028);农业部公益性行业科研专项(201303018)

摘　　要：[目的]研究安徽省辣椒疫霉(Phytophthora capsici Leonian)的致病力与多聚半乳糖醛酸酶(PG)活性的关系。[方法]采用平板法定性检测辣椒疫霉产生的果胶酶活性,并对不同致病力菌株的PG活性的变化进行分析。[结果]辣椒疫霉均可产生果胶酶,形成水解圈。供试菌株接种辣椒苗复壮后的PG活性高于复壮前;强致病力菌株的PG活性明显高于弱致病力菌株;供试菌株的PG活性均在培养后第7天达到峰值。[结论]PG活性的高低与辣椒疫霉菌致病力的强弱相关,是该菌的致病相关酶。[Objective] The effect of activities of polygalacturonase（ PG） on pathogenicity in Phytophthora capsici was investigated. [Method] The productivity and activity changes on pectinase and PG of P. capsici were analyzed according to the methods of hydrolyzed circle and liquid cultivation,respectively. [Result] The results showed that all isolates of P. capsici could produce pectinase and form hydrolytic circle.The PG activity of isolate obtained from post-inoculation on pepper seedlings was higher than that of pre-inoculation. PG activity of strong pathogenic isolate was obviously higher than that of weak pathogenic isolate. PG activities of all isolates were the highest in the seventh day after cultivation. [Conclusion] The difference in PG activity was related to the pathogenicity of P. capsici and PG was a pathogenic cell wall degradation enzyme in P. capsici.

关 键 词：辣椒疫霉 多聚半乳糖醛酸酶 致病力 

分 类 号：S436.418.19[农业科学—农业昆虫与害虫防治]