河北区试小麦品种(系)DNA指纹图谱构建及遗传差异分析  

Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Wheat Varieties(Lines) in Regional Test of Hebei

作　　者：李宏博[1,2] 庞斌双[2] 刘丽华[2] 刘阳娜[2] 赵昌平[2] 陈景堂[1] 

机构地区：[1]河北农业大学农学院,保定071001 [2]北京市农林科学院杂交小麦工程技术研究中心,杂交小麦分子遗传北京市重点实验室,北京100097

出　　处：《生物技术通报》2015年第6期93-99,共7页Biotechnology Bulletin

基　　金：国家科技支撑计划(2014BAD01B09,2013BAD04B01);北京市农林科学院科技创新重大专项(KJCX20140421,KJCX20140202)

摘　　要：采用42个SSR标记为2009-2013年河北省区域试验的70份小麦品种(系)构建DNA指纹图谱,进行遗传差异分析,为小麦品种改良和种质资源创新提供参考。结果表明,42个SSR标记在70份品种(系)中共检测到303个等位变异,单个SSR位点的平均等位变异为7.21个,PIC值平均0.69;基因多样性平均0.73,遗传相似系数变化范围为0.05-1.00,平均0.28;表明参加河北省区域试验的品种(系)间存在不同程度的遗传差异。聚类分析把70份品种(系)划分为4大类,6个亚类,表明同一育种单位或地区品种(系)遗传背景相近,应该加大外来小麦种质资源的引进和利用力度。In order to provide a reference for improving and innovating breeding materials, we used forty two pairs of SSR primers to construct DNA fingerprints and detect genetic diversity among 70 wheat varieties（lines）, which participated in regional test of Hebei in 2009-2013. The results showed that 303 allelic were revealed on 42 SSR loci, 7.21 alleles were detected for each SSR locus, and the average PIC（polymorphism information content）value was 0.69. Moreover, the average gene diversity was 0.73, and the genetic similarity varied from 0.05 to 1.00 with the average of 0.28. These results indicated that there is a large genetic difference between the wheat varieties（lines）in regional test of Hebei. The cluster analysis divided the 70 wheat varieties（lines）into four groups and six subgroups by UPGMA method. These results show that the wheat varieties（lines）have similar genetic background in the same breeding institute or region. We should strengthen the introduction and utilization of foreign wheat germplasm resources.

关 键 词：小麦 SSR标记 DNA指纹图谱 遗传差异 

分 类 号：S512.1[农业科学—作物学]