油菜素内酯基因BAS1根系表达载体的构建及烟草的遗传转化  

Construction of Root Expression Vector of Brassinosteroid Gene BAS1 and Genetic Transformation into Tobacco

作　　者：束红梅[1] 郭书巧[1] 巩元勇[1] 倪万潮[1] 

机构地区：[1]江苏省农业科学院经济作物研究所,农业部长江下游棉花与油菜重点实验室,南京210014

出　　处：《生物技术通报》2015年第6期106-110,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31201139);国家转基因专项(2011ZX08005-001);江苏省自主创新基金项目[CX(14)2065]

摘　　要：为使油菜素内酯基因BAS1在根系特异表达。首先构建含有根系启动子Tob RB7的根系表达载体p CAMBIA2301-RB7-rbc。再将获得的油菜素内酯失活基因BAS1与其整合,得到BAS1基因的根系特异表达载体p CAMBIA2301-RB7-BAS1-rbc,通过农杆菌侵染转入烟草。经PCR和RT-PCR验证,目的基因BAS1已成功转入烟草,并在根系表达,转基因植株中根系油菜素内酯受体激酶基因BRI1的表达受到影响。In order to allow brassinosteroid geneBAS1overexpress in root, the root expression vector pCAMBIA2301-RB7-rbc with root promoter TobRB7 was constructed.Then inactivated gene BAS1 was integrated into the root overexpression vector pCAMBIA2301-RB7-BAS1-rbc, and they were transformed into tobacco byAgrobactrium tumefaciems infection. With the verification by PCR and RT-PCR, the target gene BAS1 was transformed into tobacco genome successfully, and expressed in root;the expression of brassinosteroid receptor kinase geneBRI1 in roots of transgenic plants was affected.

关 键 词：BAS1基因 根系表达载体构建 转基因植株 表达 

分 类 号：Q943.2[生物学—植物学]