一株好氧反硝化-异养硝化菌的筛选及脱氮特性研究  

Screening and Denitrification Characteristics of an Aerobic Denitrifying-Heterotrophic Nitrification Bacterium

作　　者：连红民[1] 邱忠平[1] 何昆明[1] 周文秀[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《生物技术通报》2015年第6期138-143,共6页Biotechnology Bulletin

基　　金：四川省科技支撑计划(2015NZ0097);2015年研究生创新实验实践项目(YC201410225);2014年西南交通大学国创项目(201410613055)

摘　　要：针对渗滤液中硝酸盐氮和亚硝酸盐氮难以转化的问题,从好氧生物反应器填埋场中筛选出一株好氧反硝化菌-异养硝化菌HN,初步鉴定该菌为假单胞菌(Pseudomonas)。通过对不同氮源的生物转化作用研究了HN的脱氮特性,并对其脱氮条件进行了优化。结果表明,在有氧条件下,以硝酸钾为唯一氮源,HN在96 h时对硝酸盐氮的去除率达到95.44%;以硫酸铵为唯一氮源时,HN在60 h时对氨氮的去除率达到85.14%;当碳源为乙醇,碳氮比为7∶1,初始p H为7.5,温度为35℃,接种量为10%时,菌株HN脱氮效率最高。Aiming at the fact that the degradation of NO3-N and NO2-N in leachate is difficult, an aerobic denitrifying bacterium HN, preliminary identified asPseudomonas, was isolated from an aerobic biological reactor landfill. Based on its degradation capacities to different nitrogen sources, we studied the denitrification characteristics of HN and optimized the conditions for denitrification. The results showed that under aerobic conditions and potassium nitrate as sole nitrogen source, the removal efficiency of nitrogen in nitrate was up to 95.44% at 96 h;when using ammonium sulfate as sole nitrogen source, HN could remove 85.14% nitrogen in ammonia at 60 h;when the carbon source was ethanol, C/N was 7∶1, initial pH was 7.5, the temperature was 35℃ and inoculation amount was 10%, HN had the highest removal efficiency.

关 键 词：生物脱氮 好氧反硝化 异养硝化 假单胞菌 

分 类 号：X703[环境科学与工程—环境工程]