甘草水提物多成分肝肠吸收代谢差异研究  

The absorption and metabolism differences of intestine and liver for multicomponent licorice water extract

作　　者：张雷[1] 潘艳丽[2] 刘洋[1] 唐明敏[1] 隗丽[1] 侯成波[1] 程笑[1] 王豪[1] 

机构地区：[1]北京中医药大学中药学院,100102 [2]中国中医科学院中医药信息研究所办公室,北京100700

出　　处：《国际中医中药杂志》2015年第7期636-640,共5页International Journal of Traditional Chinese Medicine

摘　　要：目的：探讨甘草水提物多成分的肝肠吸收代谢差异。方法利用液质联用技术对甘草水提物的主要成分进行鉴别，并利用在体封闭肠环模型研究大鼠小肠和肝脏吸收代谢的差异。结果甘草水提物中共鉴别出13种主要化学成分。甘草水提物中部分成分可透过肠壁吸收，且在吸收过程中伴随着成分代谢，在血浆样品中共检测到14种新生代谢产物。肝脏可代谢甘草水提物中多种成分，且较肠吸收代谢过程产生更多的代谢产物。结论肝脏是甘草水提物多成分代谢的主要器官，肠道具有一定代谢作用。小肠和肝脏存在明显的吸收代谢差异。Objective This paper was to study the absorption and metabolism differences of intestine and liver for multicomponent licorice.Methods The components were identified with the UPLC-MS/MS. In situ closed-loop method was used to carry out the comparative experiments of absorption and metabolism differences between intestine and liver.Results 13 components were identified by UPLC-MS/MS. The absorption and metabolism results indicated some components in licorice water extract could be absorbed into blood and metabolism happened during this process. 14 metabolites were detected in the plasma sample. The hepatic metabolism results indicated many components could experience complex metabolism and more metabolites could be generated.Conclusions Liver was the major metabolism organ for licorice water extract and some components could be metabolized along with the absorption process in intestine. The absorption and metabolism differences between intestine and liver were significant.

关 键 词：甘草水提物 超高效液相色谱-串联质谱 肝肠吸收代谢差异 封闭肠环 

分 类 号：R285.5[医药卫生—中药学]