基于金纳米簇的Co^(2+)离子检测比率荧光探针的研究  

Ratiometer fluorescence probe based on gold-nanoclusters for the detection of Co^(2+) ion

作　　者：尹苹苹[1] 殷琦[1] 王嘉博[1] 李美丽[1] 孙国英[1] 

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012

出　　处：《分子科学学报》2015年第3期222-228,共7页Journal of Molecular Science

基　　金：国家自然科学基金青年基金资助项目(21003013);吉林省自然科学基金资助项目(201215126)

摘　　要：通过FITC与BSA的碳酰胺化反应将FITC连接到牛血清白蛋白稳定的金纳米簇(Au-BSA NCs)上,获得了一种新型的比率荧光探针Au-BSA-FITC.该荧光探针能够高效、灵敏的检测水溶液中的Co2+离子,检测限低至10nmol/L.此检测限比国家《生活饮用水卫生标准GB5749-2006》中允许的最高Co2+离子含量(1.0mg/L)低3个数量级.重要的是该荧光比率探针能够实现实际水样中Co2+离子的检测,且在细胞检测与成像等方面也具有潜在的应用价值.Bovine albumin stabilized-Gold-Nanoclusters（Au-BSA NCs）were synthesized,and then modified with TITC by carbon amidation reaction between FITC and BSA to obtain a novel ratiometric fluorescence probe Au-BSA-FITC.Under the optimized experimental conditions,this ratiometric fluorescence probe could efficient and sensitive detection of Co2＋in the aqueous solution with a detection limit as low as 10nmol/L,which was approximately 3orders of magnitude lower than the maximum level Co2＋in the drinking water permitted by the Standards for Drinking Water Quality GB5749-2006.Furthermore,several real water samples spiked with Co2＋could be successfully analyzed using this nanoprobe.

关 键 词：金纳米簇 比率荧光探针 Co2+离子 检测 实际水样 

分 类 号：O652[理学—分析化学]