盐胁迫对小麦代换系渗透调节物质的影响及染色体效应  

The Effects of Salt Stress on Osmoregulation Substance and Chromosome of Wheat Substitution Lines

作　　者：靖姣姣[1,2] 张颖[1,2] 白志英[1,2] 李存东 

机构地区：[1]河北农业大学生命科学学院,保定071000 [2]河北省作物生长调控实验室,保定071000

出　　处：《植物遗传资源学报》2015年第4期743-750,共8页Journal of Plant Genetic Resources

基　　金：河北省自然科学基金项目(C2011204016)

摘　　要：以中国春-Synthetic 6x小麦染色体代换系及其亲本为材料,设置对照(0 mmol/L Na Cl)和盐胁迫(150 mmol/L Na Cl)2个处理,通过测定不同盐处理条件下代换系幼苗渗透调节物质Na+、K+、可溶性糖、可溶性蛋白及脯氨酸含量变化,探讨盐胁迫对小麦代换系幼苗渗透调节物质的影响,并对其调控相关特性的基因进行染色体定位。结果表明,在盐胁迫条件下,小麦代换系的无机渗透调节物质Na+、K+含量明显升高,有机渗透调节物质可溶性糖、可溶性蛋白及脯氨酸含量显著增加。对相关性状的染色体定位分析表明,Synthetic 6x的1A、2A、3B、7B、1D、4D和7D染色体上存在抑制Na+含量升高的基因,4A、7A染色体上可能存在使K+含量升高的基因;6A、7A和7D染色体上可能存在使可溶性糖含量升高的基因,1A、2A、4A和6A染色体上可能存在使可溶性蛋白含量升高的基因,7A、6D染色体上可能存在使脯氨酸含量升高的基因。即Synthetic 6x的第4、7染色体(4A、4D;7A、7D)上可能含有调控无机调节物质的基因,第6染色体上(6A、6D)可能含有调控有机渗透调节物质的基因。Wheat substitution lines between Chinese Spring and Synthetic 6x under the treatments of control（ 0 mmol/L Na Cl） and salt stress（ 150 mmol/L Na Cl） were studied to research the effect of salt stress on the changes of Na^＋content,K^＋content,soluble sugar content,soluble protein content,proline content and to locate the genes controlling the relative characters. The results showed that contents of K^＋and Na^＋increased under salt treatment comparing to control. The organic osmotic substance of wheat substitution lines also increased significantly under salt tolerance. Chromosome location of relative characters showed that the genes inhibiting Na^＋content might be located on 1A,2A,3B,7B,1D,4D and 7D chromosome and increasing K^＋content might be existed on 4A and 7A chromosome of Synthetic 6x,genes promoting soluble sugar content might be located on 6A,7A,and 7D chromosome and increasing soluble protein content might be exist on 1A,2A,4A,and 6A chromosomes and the genes promoting proline content might be located on 7A and 6D chromosome of Synthetic 6x. In sum,the fourth and seventh chromosomes（ 4A,4D; 7A,7D） Synthetic 6x might contain the genes regulated inorganic substances and the sixth chromosome（ 6A,6D） might contain organic osmotic regulation genes.

关 键 词：小麦代换系 盐胁迫 渗透调节 染色体效应 

分 类 号：S512.1[农业科学—作物学]