高效液相色谱法测定骨疏灵颗粒中淫羊藿苷含量  

Determination of icariin in gushuling granule by HPLC

作　　者：俞吉[1,2] 朱裕林[3] 桑冉[3] 屈彪[1] 陈卫东[1] 

机构地区：[1]安徽中医药大学药学院,合肥230031 [2]安徽省淮南市朝阳医院药剂科,安徽淮南232001 [3]蚌埠医学院第一附属医院药剂科,安徽蚌埠233004

出　　处：《实用药物与临床》2015年第6期691-693,共3页Practical Pharmacy and Clinical Remedies

基　　金：安徽省自然科学基金资助项目(1208085MH127)

摘　　要：目的建立HPLC法测定骨疏灵颗粒中淫羊藿苷的含量。方法采用Cosmosil C18（250 mm×4.6 mm,5μm）为色谱柱,流动相：乙腈-水（30∶70）,流速：1.0 m L/min,检测波长：270 nm。结果淫羊藿苷在0.01-0.25 mg/m L范围内呈良好的线性关系（r=0.999 9）,回收率为99.99%（RSD=0.75%,n=6）。结论本方法简单、准确,灵敏度高,重现性好,可用于骨疏灵颗粒中淫羊藿有效成分淫羊藿苷的含量测定。Objective To estabilish a quantitative method for icariin in gushuling granule by HPLC. Methods HPLC was performed on Cosmosil C18（ 250 mm × 4. 6 mm,5 μm）. The mobile phase consisted of acetonitrile-water（ 30∶ 70） at a flowrate of 1. 0 m L /min,the UV detection wavelength was 270 nm. Results The linear range of icariin was in the range of 0. 01 ~ 0. 25 mg / m L（ r = 0. 999 9）,the average recovery rate was 99. 99% with RSD = 0. 75%（ n =6）. Conclusion The method is simple and accurate,it can be used for the quality control of icariin in gushuling granule.

关 键 词：骨疏灵颗粒 淫羊藿 淫羊藿苷 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学]