GC法测定标准桃金娘油肠溶胶囊中3种成分的含量  

Content determination of three components in myrtol standardized enteric coated soft capsules by GC

作　　者：张勇[1] 张慧[1] 高桂花[2] 

机构地区：[1]辽宁中医药大学,大连116600 [2]济宁医学院药学院,山东日照276826

出　　处：《实用药物与临床》2015年第6期699-702,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立测定标准桃金娘油肠溶胶囊中α-蒎烯、柠檬烯和桉叶油醇的含量测定方法。方法采用Rtx-1701毛细管柱（30 m×0.32 mm×0.25μm）,进样口温度200℃,火焰离子化检测器,测器温度220℃,柱温90℃,氮气为载气,流速1.0 m L/min,进样量1μL,外标法测定含量。结果α-蒎烯进样浓度在10.09~90.79μg/m L,回归方程为y=24 163 x＋39 804,r=0.999 8;柠檬烯进样浓度在16.82~151.3μg/m L,回归方程为y=31 699 x＋1 555 748,r=0.999 7;桉叶油醇进样浓度在41.23~371.1μg/m L,回归方程为y=7 416.8 x＋2 367 875.5,r=0.999 7。α-蒎烯的平均回收率为99.36%,RSD为1.21%;柠檬烯的平均回收率为99.14%,RSD为1.03%;桉叶油醇的平均回收率为100.22%,RSD为0.87%。结论该方法简便、准确、灵敏、重现性好,可作为标准桃金娘油肠溶胶囊中主要成分的含量测定方法。Objective To establish a method for determination of α-pinene,limonene and eudesmol in myrtol standardized enteric coated soft capsule. Methods Rtx-1701 capillary column（ 30 m × 0. 32 mm × 0. 25 μm） was adopted,inlet temperature was 200 ℃,Flame Ionization Detector（ FID） with temperature of 250 ℃,the column temperature was 90 ℃ with nitrogen as the carrier gas,the flowrate was 1 m L / min,injection volume was 1 μL. Results The linear ranges of α-pinene,limonene and eudesmol were 10. 09 ~ 90. 79 μg / m L（ y = 24 163 x ＋ 39 804,r = 0. 999 8）,16. 82 ~ 151. 3 μg / m L（ y = 31 699 x ＋ 1 555 748,r = 0. 999 6）,41. 23 ~ 371. 1 μg / m L（ y = 7 416. 8 x ＋ 2 367 875. 5,r = 0. 999 7）; the mean recovery rates of the three components were 99. 36%（ RSD = 1. 21%）,99. 14%（ RSD =1. 03%）,100. 22%（ RSD = 0. 87%）,respectively（ n = 9）. Conclusion The method is simple,accurate,sensitive,reproducible,it can be used to determine the main contents in myrtol standardized enteric coated soft capsule.

关 键 词：标准桃金娘油肠溶胶囊 Α-蒎烯 柠檬烯 桉叶油醇 含量测定 

分 类 号：O657.71[理学—分析化学] TQ460.72[理学—化学]