人血浆中吗啡血药浓度的测定  

Determination of Morphine Concentration in Human Plasma by HPLC

作　　者：丁信园[1] 金星[1] 陈芳[1] 孙坚彤[1] 张学农[2] 

机构地区：[1]南京医科大学附属苏州医院,江苏苏州215001 [2]苏州大学药学院,江苏苏州215123

出　　处：《抗感染药学》2015年第3期342-344,共3页Anti-infection Pharmacy

基　　金：苏州市科技发展计划应用基础研究项目(编号:SYSD2013148);南京医科大学科技发展基金项目(编号:2013NJMU216)

摘　　要：目的：建立HPLC法测定人血浆中吗啡浓度的方法。方法：采用高氯酸为蛋白沉淀剂，以及采用色谱枉为、vaters Spherisorb C18 ODS柱，流动相为0．05mol／L磷酸二氢钾溶液．0．0025mol／L庚烷磺酸钠水溶液-乙晴（5：5：2），柱温为30℃，流速为0．5mL／min，检测波长为220nm，进样量为20μL。结果：血浆中吗啡浓度是在1．0～100μg／mL范围内其线性关系良好（r=0．9994）；最低检出限为0-3μg／mL；低、中、高3种吗啡浓度的回收率分别为98．70％，113．71％和96．51％：日内RSD分别为3．39％，4．96％和2．27％；日间RSD分别为7．64％，5．74％和3．18％。结论：该方法操作简便、快速，可适用于人血浆中吗啡浓度的测定。Objective： To establish a method to determine rapidly morphine in human palsma by HPLC. Methods： The separation was performed on Waters Spherisorb C18 column with 0.05 mol/L potassium dihydrogen phosphate-0.002 5 mol/L sodium heptanesulfonate-acetonitrile（5 ： 5： 2） as mobile phase using perchloric acid as protein precipitant. The column temperature was 30 ℃, the flow rate was 0.5 mL/min, the detection wavelength was 220 nm, and the sam- ple volume was 20 μL. Results： The calibration curve of morphine was linear within the range of 1.0-100 μg/mL with good linearity（t=0.999 3 ）. The lowest detection limit was 0.3 μg/mL. The recovery was 98.70%, 113.71% and 96.51% with intra-day RSD of 3.39%, 4,96% and 2.27% and inter-day RSD of 7.64%, 5.74% and 3.18% for low, medium and high concentration, respectively. Conclusion： This method is simple, rapid and stable for the determination of morphine concentration in human plasma.

关 键 词：吗啡 高效液相色谱法 高氯酸 血浆浓度 

分 类 号：R996[医药卫生—毒理学]