针刺干预对脑缺血大鼠脑组织低氧诱导因子-1α蛋白和mRNA表达的影响  

Effects of acupuncture on hypoxia inducible factor-1α and local cerebral blood flow in rats with focal cerebral ischemia

作　　者：朱在卿[1] 许贵刚[1] 郭艳丽[1] 马斌[1] 卢岩[2] 

机构地区：[1]武警山东总队医院神经内科,济南250014 [2]山东中医药大学针灸推拿学院,济南250355

出　　处：《武警医学》2015年第6期551-554,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金青年项目(81102652);山东省自然科学基金(ZR2009CQ007)

摘　　要：目的观察针刺干预对脑缺血大鼠脑组织缺氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)蛋白及mRNA表达的影响,探讨针刺对缺血性脑血管疾病的防治机制。方法建立局灶性脑缺血大鼠模型,随机分为正常组、假手术组、模型组、针刺组(各8只大鼠),应用蛋白印迹及实时荧光定量检测针刺对局灶性脑缺血大鼠脑组织HIF-1α蛋白及mRNA表达的变化。结果正常组和假手术组大鼠比较HIF-1α蛋白及mRNA表达无明显变化,模型组大鼠HIF-1α蛋白及mRNA表达上调,差异无统计学意义;针刺组大鼠HIF-1α蛋白及mRNA表达高于模型组(0.567±0.058 vs 0.315±0.118;1.593±0.102vs 1.193±0.259),差异有统计学意义(P<0.05)。结论脑缺血后大鼠脑组织HIF-1α蛋白及mRNA表达增强,针刺可以上调其表达从而发挥脑保护作用。Objective To observe the effect of acupuncture on hypoxia inducible factor -1αand local cerebral blood flow in rats with focal cerebral ischemia.Methods Wistar rats were randomly divided into four groups：normal group, sham-operated group, model group and electroacupuncture（ EA） group.Focal cerebral ischemia rat models were developed by middle cerebral artery occlu-sion（ MCAO ） with thread embolus.Electroacupuncture was applied to bilateral PC 6 and LI11 after MCAO.The expression of hypoxia inducible factor-1αwas examined in MCAO rats by Western blot and real-time quantitative PCR （ qPCR ） .Results Acupuncture could promote the recovery of neurological function in MCAO rats.Western blot and real-time qPCR showed that acupuncture promoted the hypoxia inducible factor-1αexpression in the ischemic brain tissue with statistical significance（ P〈0.05） .Conclusion Acupunc-ture promotes hypoxia inducible factor-1αexpression in rats with focal cerebral ischemia.All the results suggest that the increased ex-pression of hypoxia inducible factor-1αin the ischemic brain tissue plays an important role in brain protection.

关 键 词：局灶性脑缺血 针刺 缺氧诱导因子-1Α 

分 类 号：R245.0[医药卫生—针灸推拿学]