高效液相色谱法测定奥扎格雷钠脂质体的包封率  

Encapsulation efficiency of ozagrel hydroehloride liposome by HPLC

作　　者：柳青 刘丽凤[1] 孙昌友[1] 李慧芳[2] 

机构地区：[1]武警山西总队医院药剂科,太原030006 [2]山西职工医学院药学系,太原030012

出　　处：《武警医学》2015年第6期584-586,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的：建立高效液相色谱法（high performance liquid chromatography，HPLC）测定奥扎格雷钠脂质体包封率的检测技术。方法采用HPLC，以C18色谱柱分离，以0．05 mol／L磷酸二氢钾溶液（ pH＝7．0）－甲醇－乙腈（80∶15∶5）为流动相，检测波长为274 nm测定。结果奥扎格雷在10～100μg／ml浓度范围内线性关系良好，r＝0．9997。日内、日间RSD均小于0．5％，方法回收率为（100．2±0．42）％，样品的平均包封率为（94．89±0．44）％。结论该测定方法简单，准确度高，灵敏度好，适合奥扎格雷钠脂质体的包封率测定。Objective To establish a high performance liquid chromatography （ HPLC） method for the encapsulation efficien-cy of ozagrel hydrochloride liposome.Methods The HPLC was used for determination.A C18 column was used with the mobile phase of 0.05 mol/L potassium dihydrogen phosphate solution （pH=7.0） -acetonitrile-methanol （80∶15∶5） at the detection wavelength of 274 nm.Results The calibration curve was linear in the concentration range of 10-100 μg/ml, r=0.9997.The inter-and intra-day RSD was less than 0.5 %.The method recoveries were （100.2 ±0.42） %.The average entrapment efficiency of tartrate in-doquinoline liposome was （94.89 ±0.44）%.Conclusions This method is simple,accurate and can be used to determine the encap-sulation efficiency of ozagrel hydroehloride in ozagrel hydrochloride liposome.

关 键 词：奥扎格雷钠 脂质体 包封率 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]