鼠疫耶尔森菌201株和201△pCD1株蛋白表达谱比较研究  

Comparative proteomic analysis of Yersinia pestis 201 and 201 △pCD1 strains

作　　者：刘红胜[1,2] 郭景玉[3] 冯尔玲[2] 王效义[3] 王恒樑[2] 宋亚军[3] 朱力[2] 

机构地区：[1]安徽大学生命科学学院,合肥230011 [2]军事医学科学院生物工程研究所,北京100071 [3]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室

出　　处：《军事医学》2015年第5期348-353,共6页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81171531,81125012,81373316);国家973计划资助项目(2011CB504901,2013CB910804)

摘　　要：目的分析缺失编码Ⅲ型分泌系统的大质粒p CD1对鼠疫耶尔森菌蛋白质表达谱的影响,探索鼠疫菌的潜在致病机制,为鼠疫疫苗研制提供新线索。方法通过双向电泳与质谱分析相结合的方法,比较鼠疫菌在26℃和37℃不同培养温度下,全菌蛋白表达谱差异,鉴定差异蛋白并对其功能进行初步分析。结果成功分离并鉴定了鼠疫菌201株和201△p CD1缺失突变株中的差异蛋白,其中26℃条件下鉴定到24个差异蛋白,37℃条件下鉴定到25个差异蛋白,内含7个由p CD1质粒编码的蛋白。结论通过比较蛋白质组学研究,发现大质粒p CD1缺失后,多种染色体编码蛋白的丰度发生了显著变化,暗示大质粒能调控染色体编码基因的表达。Objective To explore the potential pathogenesis of Yersinia pestis and provide new clues for vaccine development through comparative proteomic analysis of wild-type and pCD1 cured strain of Yersinia pestis 201.Methods Differentially expressed proteins at 26℃ and 37℃ were separated and identified using two-dimensional electrophoresis coupled with mass spectrometry .Results A total of 24 differently expressed proteins were successfully identified from the samples of bacteria grown at 26℃ and 25 proteins at 37℃.Among these, 7 proteins were encoded by pCD 1 plasmid. Conclusion Through comparative proteomic research, we have found that the abundance of several proteins can be dramatically changed when the large plasmid pCD 1 is missing,suggesting that the plasmid can regulate the expression of many genes located in the chromosome .

关 键 词：耶尔森菌 鼠疫 Ⅲ型分泌系统 电泳 凝胶 双向 蛋白表达谱 质谱分析法 

分 类 号：R378.61[医药卫生—病原生物学]